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SALSA digitalMLPA Probemix D007 ALL

Acute Lymphoblastic Leukemia

Region: Various

digitalMLPA | New

General information
The SALSA digitalMLPA Probemix D007-A1 Acute Lymphoblastic Leukemia is a research use only (RUO) assay for the detection of deletions, gains or amplifications in 52 specific target genes and three chromosomal regions (5q, iAMP21 and PAR1), which are associated with acute lymphoblastic leukemia (ALL) as mentioned in Table 2.
This assay is for use with genomic DNA isolated from peripheral whole blood or bone marrow specimens and is not intended to be used with genomic DNA extracted from formalin-fixed paraffin-embedded or fresh-frozen tumour materials. CNAs detected with the D007 Acute Lymphoblastic Leukemia Probemix should be confirmed with a different technique or with conventional MLPA probemixes when possible. In particular, CNAs detected by only a single probe always require confirmation by another method. The majority of defects in the genes included in this D007 Acute Lymphoblastic Leukemia Probemix are deletions, gains or amplifications, but point mutations can occur which will not be detected by digitalMLPA. It is therefore recommended to use this assay in combination with sequence analysis for the gene(s) of interest.
ALL is the most common childhood cancer and includes multiple genetically heterogeneous subtypes of malignant clonal expansions of immature T- or B-cells. Although current treatment regimens have resulted in 5-year event-free survival rates of >90% in children, disease relapse is associated with a poor outcome (Roberts and Mullighan 2015). Genetic alterations in the different subtypes of ALL are well characterised and include gross chromosomal aberrations such as hyper-/hypodiploidy, but also fusion genes, gene deletions, gains and amplifications. The digitalMLPA Probemix D007-A1 Acute Lymphoblastic Leukemia is designed to detect these key genetic copy number alterations in childhood ALL.

This SALSA digitalMLPA Probemix is not CE/FDA registered for use in diagnostic procedures. Purchase of this product includes a limited license for research purposes.

Probemix content
A total number of ~760 probes is included in this D007-A1 Acute Lymphoblastic Leukemia Probemix, consisting of:
- 347 target probes detecting copy number alterations involved in ALL (Table 2 of the product description).
- 249 karyotyping probes, covering most chromosome arms at the centromeres, middle of the chromosome arms and telomere regions. No probes are present for the short p-arm of the five acrocentric chromosomes (13, 14, 15, 21 and 22). See Table 3 of the product description for all chromosomal regions and genes included.
- More than 160 control probes and fragments: these include probes to aid in normalisation in case of copy number changes in tumour samples, probes for sample identification and probes for detection of errors or deviations when performing digitalMLPA assays, impurities in and fragmentation of the DNA samples, ligase and polymerase activity and extent of hybridisation.

More information on the location and warnings of the probes present in this probemix can be found in the Probe Information File (PIF) available at www.mrcholland.com.

More information
D007 product flyer Explore digitalMLPA panels

Order Items

Probemix

Item no.
Description
Technology
Price
D007-025R
SALSA digitalMLPA Probemix D007 ALL – 25 rxn
€ 725.00
D007-050R
SALSA digitalMLPA Probemix D007 ALL – 50 rxn
€ 1425.00
D007-100R
SALSA digitalMLPA Probemix D007 ALL – 100 rxn
€ 2800.00

Required Reagents (Sold Separately)

Item no.
Description
Technology
Price
DRK01-IL
SALSA digitalMLPA Reagent Kit – 100 rxn
€ 316.00
DRK05-IL
SALSA digitalMLPA Reagent Kit – 500 rxn
€ 1455.00
DRK20-IL
SALSA digitalMLPA Reagent Kit – 2000 rxn
€ 5590.00
BP01-IL
SALSA Barcode Solutions Plate 01 – 768 rxn (barcode solutions 1-96)
€ 1595.00
BP02-IL
SALSA Barcode Solutions Plate 02 – 768 rxn (barcode solutions 97-192)
€ 1595.00

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First released on Nov 03, 2022