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P037 CLL-1

SALSA MLPA Probemix P037 CLL-1 detects copy number variations in various genes and regions implicated in B-cell chronic lymphocytic leukemia (B-CLL).

Specifications

Contents: 54 MLPA probes, including 41 probes for the 2p, 6q, 8p/q, 9p21, 11q, 12p/q, 13q and 17p chromosomal regions.

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on B-cell chronic lymphocytic leukemia (B-CLL).

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

P038 CLL-2

Contains probes for 11q, 12p/q, 13q14, TP53 & PTEN genes and probes specific for NOTCH1 p.P2514*fs, SF3B1 p.K700E and MYD88 p.L265P point mutations.

P040 CLL

Contains probes for a selected target genes and regions from P037 and P038.

P041 ATM-1

Contain more probes for ATM on 11q.

View all

General information

SALSA MLPA Probemix P037 CLL-1 is a research use only (RUO) assay for the detection of deletions or duplications in various genes and chromosomal regions implicated in B-cell chronic lymphocytic leukemia (B-CLL) such as: 2p (MYCN, ALK, REL), 6q (TNFAIP3), 8p (TNFRSF10A/B), 8q (EIF3H, MYC), 9p21 (CDKN2A/B), 11q (ATM), chromosome 12, 13q14 (MIR15A, DLEU2/7) and 17p (TP53).

B-CLL is the most common hematologic neoplasm in Western countries and results in the progressive accumulation of morphologically mature but functionally incompetent CD5(+) CD23(+) B lymphocytes in bone marrow, blood, spleen and lymph nodes of the affected person. Chromosomal translocations are rare events in B-CLL. Copy number changes of certain chromosomal regions are however frequent and some are associated with disease prognosis.

More information is available at https://www.ncbi.nlm.nih.gov/books/NBK470433/

SALSA MLPA Probemix P038 CLL-2 contains more probes for the 11q region and different probes for chromosome 12, 13q14 and the TP53 gene. Moreover, it contains probes targeting the PTEN gene, 14q, chromosome 19, and probes specific for NOTCH1 p.P2514Rfs*4, SF3B1 p.K700E and MYD88 p.L265P point mutations. SALSA MLPA Probemix P040 CLL contains a selection of target genes and regions from P037 and P038 for the detection of copy number determination of 11q, chromosome 12, 13q14 and 17p13.

Regulatory status

SALSA MLPA Probemix P037 CLL-1 is for research use only (RUO) in all territories.

Product documentation

Version B2

Version B1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P037-025R
SALSA MLPA Probemix P037 CLL-1 – 25 rxn
MLPA
€ 286.00
P037-050R
SALSA MLPA Probemix P037 CLL-1 – 50 rxn
MLPA
€ 560.00
P037-100R
SALSA MLPA Probemix P037 CLL-1 – 100 rxn
MLPA
€ 1096.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 348.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 348.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6152.00

Other products

These optional accessories can be ordered separately.

Item no.
Description
Technology
Price
SMR05
SALSA FFPE Solution (SFS) – 50 rxn (10 ml)
MLPA MS-MLPA
€ 45.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

The commercially available positive samples below can be used with the current (B2) version of this product.

Chromosome 2

Chromosome 6

  • Coriell NA01221: Heterozygous duplication affecting the probes for AIM1 and SEC63.
  • Coriell NA06802: Heterozygous deletion affecting the probes for IGF2R and PARK2.
  • Coriell NA07994: Heterozygous duplication affecting the probes for TNFAIP3, LATS1, IGF2R and PARK2.
  • Coriell NA09367: Heterozygous duplication affecting the probes for SEC63 and TNFAIP3.

Chromosome 8

Chromosome 9

Chromosome 11

Chromosome 12

  • Coriell NA07891: Heterozygous duplication affecting the probe for CHFR.
  • Coriell NA07981: Heterozygous triplication/ homozygous duplication affecting the probes for CCND2 and LRMP.
  • Coriell NA08035: Heterozygous duplication affecting the probes for CCND2 and LRMP.

Chromosome 13

  • Coriell NA05832, Coriell NA09711: Heterozygous duplication affecting the probes for RB1, FNDC3A, KCNRG, MIR15A, DLEU2, DLEU7 and ATP7B.
  • Coriell NA13721, Coriell NA14164: Heterozygous deletion affecting the probes for RB1, FNDC3A, KCNRG, MIR15A, DLEU2, DLEU7 and ATP7B.
  • DSMZ ACC-765 (HG-3): Heterozygous deletion affecting the probes for RB1, FNDC3A and ATP7B. Homozygous deletion affecting the probes for KCNRG, MIR15A, DLEU2 and DLEU7.

Various

  • Coriell NA02819: Heterozygous duplication affecting the probes for CDKN2A and CDKN2B. Heterozygous deletion affecting the probe for CHFR.
  • DSMZ ACC-220 (KASUMI-1): Gain affecting the probes for MYC. Heterozygous deletion affecting the probes for CDKN2A, CDKN2B and TP53. Some of the reference probes are also affected by copy number alterations.
  • DSMZ ACC-600 (HNT-34): Heterozygous deletion affecting the probes for AIM1, SEC63, TNFAIP3, LATS1, IGF2R and PARK2. Gain affecting the probes for EIF3H and MYC.

Resources

General MLPA resources

MLPA education

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