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P327 iAMP21-ERG

SALSA MLPA Probemix P327 iAMP21-ERG detects copy number variations in genes and regions on chromosome 21 associated with acute lymphoblastic leukemia (ALL), myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML).

Specifications

Contents: 59 MLPA probes, including 13 probes for the ERG region (covering all exons of transcript variants 1-6 and 8), 6 probes for RUNX1, and 27 probes for the 21q11.2 - 21q22.3 region.

Tissue: human genomic DNA.

Application: research on acute lymphoblastic leukemia (ALL), myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML).

For research use only (RUO). Not for use in diagnostics.

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Key related products

ME024 9p21 CDKN2A/2B region

Contains probes for the 9p21 region, including CDKN2A and CDKN2B genes for detection of both copy number and methylation status.

P018 SHOX

Contains probes for the SHOX gene and Xp22 regions.

P047 RB1

Contains probes for 26 out of 27 exons of the RB1 gene.

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General information

The SALSA MLPA Probemix P327 iAMP21-ERG is a research use only (RUO) assay for the detection of deletions, duplications or amplifications of specific sequences on chromosome 21, which are associated with acute lymphoblastic leukemia (ALL), myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML).

ALL is a heterogeneous disease at the cytogenetic level. This can be utilized in defining clinically significant genetic subgroups to aid in risk stratification and in the treatment selection of ALL. Intrachromosomal amplification of chromosome 21 (iAMP21) is a recurrent chromosomal abnormality detected in ~2% of childhood B-lineage ALL patients (Harrison 2015). iAMP21 is more common in older children and adolescents with ALL (Soulier et al. 2003). Although the level and extent of the amplified region on chromosome 21 seems to vary between ALL patients, the common region of amplification contains the RUNX1 (AML1) gene, at 21q22.12. Clinical studies have shown that ALL patients with iAMP21 have an increased risk of relapse and significantly inferior survival compared to patients without this genetic change (Robinson et al. 2003; Moorman et al. 2010; Harrison 2015). Thereby ALL patients with iAMP21 are suggested to be treated with a more intense regimen (Moorman et al. 2013).

Similar chromosome 21 amplifications have also been reported in patients with myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML), and are suggested to associate with poor survival (Marcucci et al. 2005; Thol et al. 2012; Weber et al. 2016).

Short intragenic deletions of ERG, at 21q22.12, are also frequently described in B-cell precursor (BCP) ALL patients. BCP-ALL patients with intragenic ERG deletion are suggested to have a favourable clinical outcome and, moreover, ERG deletion is suggested to define a subgroup of superior outcomes among patients with IKZF1 ∆4-7 deletions (Clappier et al. 2014; Zaliova et al. 2014).

Regulatory status

SALSA MLPA Probemix P327 iAMP21-ERG is for research use only (RUO) in all territories.

Product documentation

Version B2

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P327-025R
SALSA MLPA Probemix P327 iAMP21-ERG – 25 rxn
MLPA
€ 296.00
P327-050R
SALSA MLPA Probemix P327 iAMP21-ERG – 50 rxn
MLPA
€ 580.00
P327-100R
SALSA MLPA Probemix P327 iAMP21-ERG – 100 rxn
MLPA
€ 1135.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

See this support article for commercially available positive samples that can be used with this product.

Resources

General MLPA resources

MLPA education

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