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SALSA MLPA Probemix P377 Hematologic Malignancies detects copy number variations associated with various hematologic malignancies.
Contents: 54 MLPA probes, including 53 probes for the chromosomal regions 2p (MYCN, ALK), 5q (MIR145, EBF1 and MIR146A), 6q, 7p12 (IKZF1), 7q, 8q24 (MYC), 9p (MTAP, CDKN2A, CDKN2B, PAX5), 10q23 (PTEN), 11q22 (ATM), 12p13 (ETV6, CCND2, MDM2), 12q, 13q14 (RB1, MIR15A, DLEU2, DLEU1), 17p13 (TP53), 17q, 18 p/q, 19 p/q and 21q22 (RUNX1). In addition, a probe for JAK2 p.V617F is included.
Tissue: human genomic DNA.
For research use only (RUO). Not for use in diagnostics.
The SALSA MLPA Probemix P377 Hematologic Malignancies is a research use only (RUO) assay, and is intended for screening DNA samples derived from blood or bone marrow for the most common and diagnostically significant copy number changes associated with hematologic malignancies, including acute lymphoblastic leukaemia (ALL), acute myeloid leukaemia (AML), chronic lymphoid leukaemia (CLL), chronic myeloid leukaemia (CML), myelodysplastic syndrome (MDS) and various lymphomas. The probemix can also be used to detect the JAK2 p.V617F point mutation which is commonly detected in myeloproliferative neoplasm (MPN). This probemix is intended to be used in combination with karyotype analysis. Suggestions on MLPA probemixes that can be used to confirm results or to get a better resolution on genes or chromosomal areas of interest can be found in Table 2 of the product description.
SALSA MLPA Probemix P377 Hematologic Malignancies is for research use only (RUO) in all territories.
SALSA Binning DNA SD068 is an artificial DNA sample with a signal for all probes in the P377 Hematologic Malignancies probemix. Inclusion of a reaction with SD068 in initial experiments and in experiments following a change in electrophoresis conditions is recommended to aid in the creation of a bin set that links peaks to the probes that produce them. Binning DNA cannot be used as a reference sample in the MLPA data analysis, and cannot be used to quantify the signals of mutation-specific probes.
A vial of SALSA Binning DNA SD068 is included with every order of the P377 Hematologic Malignancies probemix, but it is possible to order additional vials separately.
For more information, see the product description.
A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).
A vial is included with every order of this probemix, but additional vials can also be purchased separately.
The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.
Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.
See this support article for commercially available positive samples that can be used with this product.