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P377 Hematologic Malignancies

SALSA MLPA Probemix P377 Hematologic Malignancies detects copy number variations associated with various hematologic malignancies.

Specifications

Contents: 54 MLPA probes, including 53 probes for the chromosomal regions 2p (MYCN, ALK), 5q (MIR145, EBF1 and MIR146A), 6q, 7p12 (IKZF1), 7q, 8q24 (MYC), 9p (MTAP, CDKN2A, CDKN2B, PAX5), 10q23 (PTEN), 11q22 (ATM), 12p13 (ETV6, CCND2, MDM2), 12q, 13q14 (RB1, MIR15A, DLEU2, DLEU1), 17p13 (TP53), 17q, 18 p/q, 19 p/q and 21q22 (RUNX1). In addition, a probe for JAK2 p.V617F is included.

Tissue: human genomic DNA.

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

ME024 9p21 CDKN2A/2B region

Contains probes for the detection of copy number and methylation changes of genes located at 9p21.3.

P037 CLL-1

Contains probes for detecting characteristic copy number changes in CLL.

P038 CLL-2

Contains probes for detecting characteristic copy number changes in CLL.

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General information

The SALSA MLPA Probemix P377 Hematologic Malignancies is a research use only (RUO) assay, and is intended for screening DNA samples derived from blood or bone marrow for the most common and diagnostically significant copy number changes associated with hematologic malignancies, including acute lymphoblastic leukaemia (ALL), acute myeloid leukaemia (AML), chronic lymphoid leukaemia (CLL), chronic myeloid leukaemia (CML), myelodysplastic syndrome (MDS) and various lymphomas. The probemix can also be used to detect the JAK2 p.V617F point mutation which is commonly detected in myeloproliferative neoplasm (MPN). This probemix is intended to be used in combination with karyotype analysis. Suggestions on MLPA probemixes that can be used to confirm results or to get a better resolution on genes or chromosomal areas of interest can be found in Table 2 of the product description.

Regulatory status

SALSA MLPA Probemix P377 Hematologic Malignancies is for research use only (RUO) in all territories.

SALSA Sample DNA for this product

SALSA Binning DNA SD068 is an artificial DNA sample with a signal for all probes in the P377 Hematologic Malignancies probemix. Inclusion of a reaction with SD068 in initial experiments and in experiments following a change in electrophoresis conditions is recommended to aid in the creation of a bin set that links peaks to the probes that produce them. Binning DNA cannot be used as a reference sample in the MLPA data analysis, and cannot be used to quantify the signals of mutation-specific probes.

A vial of SALSA Binning DNA SD068 is included with every order of the P377 Hematologic Malignancies probemix, but it is possible to order additional vials separately.

For more information, see the product description.

Product documentation

Version B1

Version A3

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P377-025R
SALSA MLPA Probemix P377 Hematologic Malignancies – 25 rxn
MLPA
€ 286.00
P377-050R
SALSA MLPA Probemix P377 Hematologic Malignancies – 50 rxn
MLPA
€ 560.00
P377-100R
SALSA MLPA Probemix P377 Hematologic Malignancies – 100 rxn
MLPA
€ 1096.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 348.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 348.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6152.00

Sample DNAs (included)

A vial is included with every order of this probemix, but additional vials can also be purchased separately.

Item no.
Description
Technology
Price
SD068
SALSA Binning DNA SD068 – 6 rxn
MLPA
€ 24.15

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

The commercially available positive samples below can be used with the current (B1) version of this product.

Chromosome 2

Chromosome 5

  • Coriell NA04371: Heterozygous duplication affecting the probes for EBF1 and MIR146A.

Chromosome 6

  • Coriell NA01221: Heterozygous duplication affecting the probe for FYN.
  • Coriell NA07994: Heterozygous duplication affecting the probes for MYB, ESR1 and SMOC2.
  • Coriell NA08386: Heterozygous deletion affecting the probe for SMOC2.
  • Coriell NA09367: Heterozygous duplication affecting the probes for FYN and MYB.

Chromosome 7

Chromosome 8

Chromosome 9

Chromosome 10

Chromosome 11

Chromosome 12

  • Coriell NA07981: Heterozygous triplication/homozygous duplication affecting the probes for CCND2 and ETV6.
  • Coriell NA08035: Heterozygous duplication affecting the probes for CCND2 and ETV6.

Chromosome 13

  • Coriell NA05832: Heterozygous duplication affecting the probes for RB1, MIR15A, DLEU2 and DLEU1.
  • Coriell NA14164: Heterozygous deletion affecting the probes for RB1, MIR15A, DLEU2 and DLEU1.

Chromosome 18

Various

  • Coriell NA12722: Heterozygous duplication affecting the probes for MTAP, CDKN2A and CDKN2B on chromosome 9. Heterozygous duplication affecting the probes for RNMT and DCC on chromosome 18.
  • Coriell NA23245: Heterozygous deletion affecting the probes for IKZF1, CDK6, RELN, MET and DPP6 on chromosome 7. Heterozygous duplication affecting the probes for MYC on chromosome 8. Positive for the JAK2 p.V617F mutation on chromosome 9.
  • Horizon Discovery HD649: Heterozygous duplication affecting the probes for IKZF1, CDK6, RELN, MET and DPP6 on chromosome 7. Positive for the JAK2 p.V617F mutation on chromosome 9. Heterozygous duplication affecting the probe for DCC on chromosome 18.
  • Horizon Discovery HD652: Heterozygous duplication affecting the probes for IKZF1, CDK6, RELN, MET and DPP6 on chromosome 7. Heterozygous duplication affecting the probe for DCC on chromosome 18.

Resources

General MLPA resources

MLPA education

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