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P414 MDS

SALSA MLPA Probemix P414 MDS detects copy number variations in various chromosomal regions with relevance in myelodysplastic syndromes (MDS).

Specifications

Contents: 46 MLPA probes, including for chromosome 3 (MLH1, GATA2, MECOM), 5q (APC, EGR1, MIR145, RPS14, SPARC, MIR146A), 7q (CDK6, SAMD9L, EPO, KMT2E, MET, EZH2), 8q (FGFR1, NCOA2, RUNX1T1, MYC, PTK2), 11q (KMT2A, TIRAP, ETS1), 12p (ETV6, CDKN1B), chromosome 17 (TP53, NF1, SUZ12, AATF), chromosome 19 (SMARCA4, PRPF31), 20q (ASXL1, SRC, HNF4A, ZMYND8) and the Y-chromosome (ZFY), and a probe for JAK2 p.V617F.

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on myelodysplastic syndromes (MDS).

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

P056 TP53

This probemix contains probes for each exon of TP53 gene.

P323 CDK4-HMGA2-MDM2

This probemix contains more probes for chromosome 12.

P335 ALL-IKZF1

This probemix contains more probes for ETV6 gene.

View all

General information

The SALSA MLPA Probemix P414 MDS is a research use only (RUO) assay for the detection of deletions or duplications in different chromosomal regions described to be of potential diagnostic or prognostic relevance in myelodysplastic syndromes (MDS), and are used in the International Prognostic Scoring System–Revised (IPSS-R) and Molecular International Prognostic Scoring System for Myelodysplastic Syndromes (IPSS-M) (Bernard E et al. 2022): chromosome 3, 5q (EGR1, MIR145, SPARC, MIR146A), 7q (EZH2), 8q (MYC), 11q (KMT2A), 12p (ETV6), chromosome 17 (TP53, NF1, SUZ12), chromosome 19, 20q (ASXL1) and Y-chromosome. This probemix can also be used to detect the presence of the JAK2 p.V617F (c.1849G>T) point mutation.

Myelodysplastic syndromes are a heterogeneous collection of hematologic disorders, which are characterized by dysplastic hematopoietic differentiation. In 30% of all cases, MDS progresses to acute myeloid leukemia (AML). There are several oncogenes and tumour suppressor genes, that have mutations or copy number alterations (CNAs) in MDS (Ogawa S. 2019), many of which can be detected with this P414 probemix.

Regulatory status

SALSA MLPA Probemix P414 MDS is for research use only (RUO) in all territories.

SALSA Sample DNA for this product

SALSA Binning DNA SD029 is an artificial DNA sample with a signal for all probes in the P414 MDS probemix. Inclusion of a reaction with SD029 in initial experiments and in experiments following a change in electrophoresis conditions is recommended to aid in the creation of a bin set that links peaks to the probes that produce them. Binning DNA cannot be used as a reference sample in the MLPA data analysis, and cannot be used to quantify the signals of mutation-specific probes.

A vial of SALSA Binning DNA SD029 is included with every order of the P414 MDS probemix, but it is possible to order additional vials separately.

For more information, see the product description.

Product documentation

Version C1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P414-025R
SALSA MLPA Probemix P414 MDS – 25 rxn
MLPA
€ 286.00
P414-050R
SALSA MLPA Probemix P414 MDS – 50 rxn
MLPA
€ 560.00
P414-100R
SALSA MLPA Probemix P414 MDS – 100 rxn
MLPA
€ 1096.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 348.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 348.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6152.00

Sample DNAs (included)

A vial is included with every order of this probemix, but additional vials can also be purchased separately.

Item no.
Description
Technology
Price
SD029
SALSA Binning DNA SD029 – 6 rxn
MLPA MS-MLPA
€ 24.15

Other products

These optional accessories can be ordered separately.

Item no.
Description
Technology
Price
SMR05
SALSA FFPE Solution (SFS) – 50 rxn (10 ml)
MLPA MS-MLPA
€ 45.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

See this support article for commercially available positive samples that can be used with this product.

Resources

General MLPA resources

MLPA education

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