Choose your country to see the products for your location

P038 CLL-2

SALSA MLPA Probemix P038 CLL-2 detects copy number variations in various genes and regions implicated in B-cell chronic lymphocytic leukemia (B-CLL).

Specifications

Contents: 51 MLPA probes, including 35 probes for 10q23 (PTEN), 11q (ATM), chromosome 12, 13q14 (RB1, DLEU1/2), 14q, 17p13 (TP53), and chromosome 19, and probes for NOTCH1 c.7541-7542delCT, SF3B1 c.2098A>G, and MYD88 c.794T>C.

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on B-cell chronic lymphocytic leukemia (B-CLL).

For research use only (RUO). Not for use in diagnostics.

Quick links

List Prices Documentation

Key related products

P037 CLL-1

Contains probes for 2p, 6q, 8p/q, 9p21, ATM, chromosome 12, 13q14 and TP53.

P040 CLL

Contains probes for 11q, 12p/q, 13q14 and TP53.

P041 ATM-1

Contain more probes for ATM on 11q.

View all

General information

The SALSA MLPA Probemix P038 CLL-2 is a research use only (RUO) assay for the detection of deletions or duplications in the genes/chromosomal regions implicated in B-cell chronic lymphocytic leukemia (B-CLL) such as: 10q23 (PTEN), 11q (ATM), chromosome 12, 13q14 (RB1, DLEU1/2), 14q, 17p13 (TP53) and chromosome 19. This probemix can also be used to detect the presence of the NOTCH1 c.7541-7542delCT (p.P2514Rfs*4), SF3B1 c.2098A>G (p.K700E) and MYD88 c.794T>C (p.L265P, also known as p.L252P) point mutations.

B-CLL is the most common hematologic neoplasm in Western countries and results in the progressive accumulation of morphologically mature but functionally incompetent CD5(+) CD23(+) B lymphocytes in the bone marrow, blood, spleen and lymph nodes of the affected person. Chromosomal translocations are rare events in B-CLL. Copy number changes of certain chromosomal regions are, however, frequent. Some of these have been found to be highly prognostic markers of this disease.

More genomic regions affected by copy number alterations (CNAs) in CLL are targeted by the P037 CLL-1 probemix containing probes for 2p (MYCN, ALK, REL), 6q (TNFAIP3), 8p (TNFRSF10A/B), 8q (EIF3H, MYC), 9p21 (CDKN2A/B), 11q (ATM), chromosome 12, 13q14 (RB1, MIR15A, DLEU2/7) and TP53. Moreover, the P040 CLL probemix contains a selection of target genes and regions from P037 and P038 allowing copy number determination of 11q, chromosome 12, 13q14 and 17p13.

More information is available at https://www.ncbi.nlm.nih.gov/books/NBK470433/

Regulatory status

SALSA MLPA Probemix P038 CLL-2 is for research use only (RUO) in all territories.

SALSA Sample DNA for this product

SALSA Binning DNA SD009 is an artificial DNA sample with a signal for all probes in the P038 CLL-2 probemix. Inclusion of a reaction with SD009 in initial experiments and in experiments following a change in electrophoresis conditions is recommended to aid in the creation of a bin set that links peaks to the probes that produce them. Binning DNA cannot be used as a reference sample in the MLPA data analysis, and cannot be used to quantify the signals of mutation-specific probes.

A vial of SALSA Binning DNA SD009 is included with every order of the P038 CLL-2 probemix, but it is possible to order additional vials separately.

For more information, see the product description.

Product documentation

Version B2

Version B1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P038-025R
SALSA MLPA Probemix P038 CLL-2 – 25 rxn
MLPA
€ 286.00
P038-050R
SALSA MLPA Probemix P038 CLL-2 – 50 rxn
MLPA
€ 560.00
P038-100R
SALSA MLPA Probemix P038 CLL-2 – 100 rxn
MLPA
€ 1096.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 348.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 348.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6152.00

Sample DNAs (included)

A vial is included with every order of this probemix, but additional vials can also be purchased separately.

Item no.
Description
Technology
Price
SD009
SALSA Binning DNA SD009 – 6 rxn
MLPA
€ 24.15

Other products

These optional accessories can be ordered separately.

Item no.
Description
Technology
Price
SMR05
SALSA FFPE Solution (SFS) – 50 rxn (10 ml)
MLPA MS-MLPA
€ 45.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

See this support article for commercially available positive samples that can be used with this product.

Resources

General MLPA resources

MLPA education

This site uses cookies to help improve your online experience. Learn more
No, thank you accept

Sign in

Don't have an account? Create one

Forgot password?

Select Your Country

Choose your country to see the products for your location