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SALSA Melt Assays: Simple, Robust Screening for Variants
Fast.

Fast

DBS card punch to results in around four hours.

Robust.

Robust

One primer set used, with minimal sensitivity to sample carryover.

Low cost

Low cost

Reagents cost around € 4 per sample; software is free.

SALSA® Melt Assays are designed for the fast and reliable screening of a small number of targets in a large number of samples. The first product in the line is SALSA® MC002 SMA Newborn Screen, with more assays planned for future release.

SALSA Melt Assay SMA Newborn Screen

SALSA MC002 SMA Newborn Screen detects the single clinically relevant nucleotide difference between SMN1 and SMN2 to identify 95-98% if SMA patients in a fast and affordable way. The assay identifies the exon 7 presence of both SMN1 and SMN2 in DNA from dried blood spot (DBS) cards crude extracts or peripheral blood, and has the added advantage that carriers are not identified.

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SALSA Melt Assay SMA Newborn Screen.

The power of SALSA Melt Assays in genetic diagnostics

We know time is critical in clinical genetic settings. SALSA Melt Assays keep time in mind, from easy lab integration, running on most melt-curve capable thermocyclers, to fast sample preparation and assay completion, to get you answers as quickly as possible.

Power of SALSA Melt Assays in genetic diagnostics.

Our technology explained

SALSA Melt Assays use a single PCR primer pair to generate amplicons that include the variant of interest. A fluorescently labelled oligonucleotide probe then binds to the amplicon in the region of the variant. Depending on the variant present, a probe will form a perfect or imperfect double-stranded amplicon–probe hybrid with a different melt temperature. Differing melt temperatures allow for variant identification.

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PCR amplification.

PCR amplification

Probe hybridisation.

Probe hybridisation

Variant identification.

Variant identification

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