Fast
DNA to results in around four hours.
Robust
One primer set used, with minimal sensitivity to sample carryover.
Low cost
Reagents cost around € 4 per sample.
SALSA Melt Assays are designed for the fast and reliable screening of a small number of targets in a large number of samples. See below our current applications.
SMA Newborn Screen
MC002 SMA Newborn Screen detects the single clinically relevant nucleotide difference between SMN1 and SMN2 to identify 95-98% of SMA patients in a fast and affordable way. The assay identifies the exon 7 presence of both SMN1 and SMN2 in DNA from dried blood spot (DBS) card crude extracts, and has the added advantage that carriers are not identified.
Lactase Persistence Genotyping
MC005 Lactase Persistence Genotyping 1 and MC006 Lactase Persistence Genotyping 2 detect single nucleotide polymorphisms (SNPs) that are located upstream of the lactase gene LCT and are associated with the genetics of lactose intolerance. The two assays extend each other's detection capability. Depending on the population being tested, the use of both products may be recommended or required.
The power of SALSA Melt Assays in genetic diagnostics
We know time is critical in clinical genetic settings. SALSA Melt Assays keep time in mind, from easy lab integration, compatibility with most melt-curve capable thermocyclers, to fast sample preparation and assay completion, to get you answers as quickly as possible.
SALSA Melt Assay technology explained
SALSA Melt Assays use a single PCR primer pair to generate amplicons that include the variant of interest (first image below). A fluorescently labelled oligonucleotide probe then binds to the amplicon in the region of the variant (second image). Depending on the variant present, a probe will form a perfect or imperfect double-stranded amplicon–probe hybrid with a different melt temperature. Differing melt temperatures allow for variant identification (third image).
PCR amplification
Probe hybridisation
Variant identification
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