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P252 NB mix 2

SALSA MLPA Probemix P252 NB mix 2 detects copy number variations in several regions on chromosomes 2 and 17 related to neuroblastoma.

Specifications

Contents: 49 MLPA probes, including 34 probes for chromosomes 2 (including the MYCN region and 2q33) and 17 (17p and 17q).

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on neuroblastoma (NB).

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

ME024 9p21 CDKN2A/2B region

Contains probes for the detection of both copy number and methylation status of genes in the 9p21 (CDKN2A and CDKN2B) region.

P056 TP53

Contains at least one probe for each exon of TP53.

P088 Oligodendroglioma 1p-19q

Contains probes for the 1p and 19q chromosomal arms, CDKN2A & CDKN2B genes and IDH1 R132H/C and IDH2 R172M/K point mutations.

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P252 NB mix 2 is shipped together with a vial of CM002 competitor mix that can be used to reduce the signal of MYCN region probes in samples with very high levels of MYCN amplification. See the product description for details and usage instructions.

General information

SALSA MLPA Probemixes P251/P252/P253 NB mix 1, 2 & 3 are research use only (RUO) assays for copy number determination of several chromosomal regions that frequently show copy number changes in neuroblastoma tumours.

Neuroblastoma (NB) is a relatively common paediatric cancer that usually occurs sporadically and frequently originates from one of the adrenal glands. NB is characterized by striking clinical heterogeneity, including cases that show spontaneous tumour regression. NB accounts up to 10% of all paediatric cancers. Several acquired genetic alterations such as amplification of the MYCN oncogene, deletions of chromosome bands 1p36 and 11q23 and unbalanced gains of 17q regions have been well-characterized and show correlation with tumour behaviour, including response to treatment. For review please see e.g. Ambros et al. (2009) and Ahmed et al. (2017).

Regulatory status

SALSA MLPA Probemix P252 NB mix 2 is for research use only (RUO) in all territories.

Product documentation

Version D1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P252-025R
SALSA MLPA Probemix P252 NB mix 2 – 25 rxn
MLPA
€ 296.00
P252-050R
SALSA MLPA Probemix P252 NB mix 2 – 50 rxn
MLPA
€ 580.00
P252-100R
SALSA MLPA Probemix P252 NB mix 2 – 100 rxn
MLPA
€ 1135.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

The commercially available positive samples below can be used with the current (D1) version of this product.

Chromosome 2

  • Coriell NA00501: Heterozygous deletion affecting the probe for TMEM18.
  • Coriell NA00945, Coriell NA09216: Heterozygous deletion affecting the probes for NBAS, DDX1 and MYCN.
  • Coriell NA01229: Heterozygous duplication affecting the probes for CFLAR, CASP8 and BMPR2.
  • Coriell NA10401: Heterozygous duplication affecting the probes for TMEM18, TPO, NBAS, DDX1, MYCN, ALK, RTN4, DYSF, RPIA, SCN1A, CFLAR, CASP8 and BMPR2.
  • Coriell NA10607: Heterozygous deletion affecting the probe for SCN1A.
  • Coriell NA10951: Heterozygous duplication affecting the probe for TPO.
  • Coriell NA11213: Heterozygous deletion affecting the probes for CFLAR, CASP8 and BMPR2.

Chromosome 17

  • Coriell NA06047: Heterozygous deletion affecting the probe for PAFAH1B1.
  • Coriell NA13031: Heterozygous deletion affecting the probe for TOB1.
  • Coriell NA16445: Heterozygous duplication affecting the probes for BIRC5, SECTM1 and TBCD.

Resources

General MLPA resources

MLPA education

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