Bisulfite-free
No harmful reagents needed.
Reliable
High gene coverage for comprehensive CNV detection.
High-throughput
Up to 384 samples in one run.
Why MS-digitalMLPA?
MS-digitalMLPA is a variant of our latest technology digitalMLPA, a powerful combination of our trusted MLPA and NGS. Combining digitalMLPA with the methylation-sensitive endonuclease HhaI allows for the detection of both DNA copy number and methylation status in one single assay.
How does MS-digitalMLPA work?
In MS-digitalMLPA, CNVs are detected using probes that are amplified after binding to their genomic targets, as in digitalMLPA. Methylation status is assessed using specialised probes that, upon hybridisation to their targets, form a recognition site for the methylation-sensitive endonuclease HhaI. When the target is unmethylated, HhaI cleaves the probe, preventing amplification. When the target is methylated, the probe is protected from digestion and can be amplified. Each probe amplicon is then quantified using read counts from a supported NGS platform.
Get started with digitalMLPA
Explore our digitalMLPA Getting started guide, which brings together all essential resources for beginners. Through a combination of videos and clear background explanations, you’ll gain insight into the technique, step‑by‑step protocol execution, data analysis and quality control, as well as links to more in‑depth resources for further learning.
Data analysis
Coffalyser digitalMLPA is our free and easy-to-use data analysis software that makes analysing digitalMLPA experiments fast and effortless. Coffalyser digitalMLPA can automatically recognise digitalMLPA sequence reads from FASTQ files and directly uses them for data analysis. Thanks to its advanced quality checks and algorithms, Coffalyser digitalMLPA can quickly detect and analyse aberrations in your samples, providing you with clear and detailed reports for every experiment.
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