Bisulfite free
Enzyme-based methylation analysis, no DNA conversion needed.
Multiplex
Detection of up to 60 DNA sequences in one single reaction.
Integrated analysis
Assess copy number and methylation in a single assay.
Why MS-MLPA?
Combining MLPA with the methylation-sensitive endonuclease HhaI allows for the detection of both DNA copy number and methylation status in one single reaction. With MS-MLPA, simultaneous semi-quantitative DNA methylation profiling of multiple targets is accomplished easily and without bisulfite treatment.
Explore our MS-MLPA applications
Inherited Disorders
Imprinting disorder profiling for Prader-Willi, Angelman and 15q11 duplication syndromes.
Fragile X syndrome associated promoter methylation profiling for male samples.
Imprinting disorder profiling for Beckwith-Wiedemann and Russell-Silver syndromes.
Imprinting disorder profiling for Albright hereditary osteodystrophy and pseudohypoparathyroidism.
Imprinting disorder profiling for UPD7 (Russell-Silver syndrome) and UPD14 (Temple & Kagami-Ogata syndromes).
Imprinting disorder profiling for Transient Neonatal Diabetes Mellitus (TNDM).
Multilocus imprinting disturbance profiling; distinguishing maternal from paternal triploidies
Tumour Profiling
Methylation profiling for 25 tumour suppressor genes.
MLH1 methylation, BRAF p.V600E point mutation and associated Lynch syndrome genome changes profiling.
Targeted glioma profiling including MGMT methylation and IDH1/2 mutation detection.
Cell cycle regulator profiling on 9p21 region associated with multiple tumour types including melanoma.
CpG Island Methylator Phenotype profiling (CIMP).
RB1 promoter and imprinted locus methylation profiling, and RB1 gene single exon level copy number detection.
Targeted methylation profiling of BRCA1, BRCA2 and RAD51C promoter regions in germline or somatic DNA.
How does MS-MLPA work?
Principle of MS-MLPA
The probes hybridise to their target sequences on the sample DNA. The HhaI enzyme is then used to digest unmethylated double-stranded probe–DNA complexes.
Complexes with unmethylated sample DNA are digested. Digested probes cannot be amplified exponentially during the multiplex PCR step, and therefore produce no signal. Complexes with methylated sample DNA are not digested by HhaI, and will generate a normal signal.
MS-MLPA reaction
The steps for MS-MLPA are highly similar to those outlined for MLPA. The most important difference is that the reaction is split into an undigested and a digested reaction after overnight hybridisation.
The undigested reaction is used for copy number determination. In the digested reaction, which is used for methylation profiling, the HhaI enzyme is added at the same time as the Ligase-65 enzyme.
Get started with MLPA
If you are new to MLPA, you may want to have a look at our guide to getting started, which lists the best resources for beginners in a stepwise fashion. Videos, e-learning modules and background explanations will help you learn more about the technique, the practical execution of the protocol, how to perform data analysis and quality control, and where to find more detailed information.
Data analysis
Coffalyser.Net is free MLPA analysis software made and supported by MRC Holland. The software can directly import raw data files, and performs advanced quality checks to increase the robustness of your results. Analysis sheets for each product can be retrieved directly from our servers, ensuring you always have access to the latest versions.
Speak to a specialist
Contact our support team or send us a message.