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SALSA MLPA Probemix P040 CLL

Chronic lymphocytic leukemia (CLL)

Region: Various

General information
The SALSA MLPA Probemix P040 CLL is a research use only (RUO) assay for the detection of deletions or duplications in the TP53 gene on 17p13, the RB1/DLEU/MIR15A-16-region on 13q14, the ATM gene on 11q22 as well the presence of trisomy 12 in DNA samples obtained from chronic lymphocytic leukemia patients.

B cell chronic lymphocytic leukemia (B-CLL) is the most common hematologic neoplasm in Western countries and results in the progressive accumulation of morphologically mature but functionally incompetent CD5(+) CD23(+) B lymphocytes in bone marrow, blood, spleen and lymph nodes of the affected person. Chromosomal translocations are rare events in B-CLL. Copy number changes of certain chromosomal regions are however frequent. Some of these have been found to be prognostic markers of this disease.

SALSA MLPA probemixes P037 and P038 contain probes for several genomic regions and genes that are recurrently imbalanced in B-CLL. This P040 probemix contains a selection of targeted genes and regions from P037 and P038.

More information is available at https://www.ncbi.nlm.nih.gov/books/NBK470433/

This SALSA MLPA probemix is not CE/FDA registered for use in diagnostic procedures. Purchase of this product includes a limited license for research purposes.

Probemix content
The SALSA MLPA Probemix P040-B2 CLL contains 52 MLPA probes with amplification products between 131 and 497 nucleotides (nt). This includes six probes for the TP53 gene on 17p13, 10 probes for the RB1/DLEU/MIR15A-16-region on 13q14, seven probes for the ATM gene on 11q22 as well 11 probes on chromosome 12. In addition, 13 reference probes are included targeting relatively copy number stable regions in various cancer types including CLL. Complete probe sequences and the identity of the genes detected by the reference probes are available in Table 3 and online (www.mrcholland.com).

This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mrcholland.com.




Order Items

Probemix

Item no.
Description
Technology
Price
P040-025R
SALSA MLPA Probemix P040 CLL – 25 rxn
€ 281.00
P040-050R
SALSA MLPA Probemix P040 CLL – 50 rxn
€ 550.00
P040-100R
SALSA MLPA Probemix P040 CLL – 100 rxn
€ 1075.00

Required Reagents (Sold Separately)

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
€ 341.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
€ 341.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
€ 1571.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
€ 1571.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
€ 6037.00

Related Products

SALSA MLPA Probemix P037 CLL-1

Contains probes for 2p, 6q, 8p/q, 9p21, ATM, chromosome 12, 13q14 and TP53.

SALSA MLPA Probemix P038 CLL-2

Contains probes for 11q, 12p/q, 13q14, TP53 & PTEN genes and probes specific for NOTCH1 p.P2514*fs, SF3B1 p.K700E and MYD88 p.L265P point mutations.

SALSA MLPA Probemix P041 ATM-1

Contain probes for all ATM exons.

SALSA MLPA Probemix P042 ATM-2

Contain probes for all ATM exons.

SALSA MLPA Probemix P047 RB1

Contains more probes for 13q14 (RB1).

SALSA MLPA Probemix P056 TP53

Contains probes for all TP53 exons.

SALSA MLPA Probemix P252 NB mix 2

Contains more probes for the 2p region.

SALSA MLPA Probemix P323 CDK4-HMGA2-MDM2

Contains more probes for chromosome 12.

SALSA MLPA Probemix P377 Hematologic Malignancies

Contains more probes for 6q, 8q, 9p, 11q, 12p/q, 13q14 and TP53.

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