General information
SALSA
® MLPA
® Probemix P037 CLL-1 is a
research use only (RUO) assay for the detection of deletions or duplications in various genes and chromosomal regions implicated in B-cell chronic lymphocytic leukemia (B-CLL) such as: 2p (
MYCN,
ALK,
REL), 6q (
TNFAIP3), 8p (
TNFRSF10A/B), 8q (
EIF3H,
MYC), 9p21 (
CDKN2A/B), 11q (
ATM), chromosome 12, 13q14 (
MIR15A,
DLEU2/7) and 17p (
TP53).
B-CLL is the most common hematologic neoplasm in Western countries and results in the progressive accumulation of morphologically mature but functionally incompetent CD5(+) CD23(+) B lymphocytes in bone marrow, blood, spleen and lymph nodes of the affected person. Chromosomal translocations are rare events in B-CLL. Copy number changes of certain chromosomal regions are however frequent and some are associated with disease prognosis.
More information is available at
https://www.ncbi.nlm.nih.gov/books/NBK470433/
SALSA
® MLPA
® Probemix P038 CLL-2 contains more probes for the 11q region and different probes for chromosome 12, 13q14 and the
TP53 gene. Moreover, it contains probes targeting the
PTEN gene, 14q, chromosome 19, and probes specific for
NOTCH1 p.P2514Rfs*4,
SF3B1 p.K700E and
MYD88 p.L265P point mutations. SALSA
® MLPA
® Probemix P040 CLL contains a selection of target genes and regions from P037 and P038 for the detection of copy number determination of 11q, chromosome 12, 13q14 and 17p13.
This product is not CE/FDA registered for use in diagnostic procedures. The SALSA® MLPA® technique is covered by US patent 6,955,901 and corresponding patents outside the US. The purchase of this product includes a license to use only this amount of product solely for the purchaser’s own use.
Probemix content
P037-B2 CLL-1 contains 54 MLPA probes with amplification products between 130 and 500 nucleotides (nt). This includes 41 probe(s) for 2p, 6q, 8p/q, 9p21, 11q, 12p/q, 13q and 17p chromosomal regions. In addition, 13 reference probes are included that target relatively copy number stable regions in various cancer types including CLL. Complete probe sequences and the identity of the genes detected by the reference probes are available in Table 3 and online (
www.mrcholland.com).
This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at
www.mrcholland.com.
Positive samples
Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the
Coriell Institute.
See
this support article for commercially available positive samples that have been tested with this product.