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ME053 BRCA1-BRCA2-RAD51C

SALSA MLPA Probemix ME053-A1 BRCA1-BRCA2-RAD51C detects copy number variations and methylation status of the BRCA1 and BRCA2 genes, as well as of the RAD51C region.

Specifications

Contents: 38 MLPA probes, including 3 probes for BRCA1, 4 probes for BRCA2 and 3 probes for the RAD51C region, which all provide information on the methylation status.

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on hereditary breast and ovarian cancer (HBOC).

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

ME001 Tumour suppressor mix

Contains (MS-)MLPA probes for the BRCA1 and BRCA2 genes, and other tumour suppressor genes.

P002 BRCA1

Contains probes for the BRCA1 gene, involved in breast and ovarian cancer syndrome.

P045 BRCA2/CHEK2

Contains probes for the BRCA2 gene, involved in breast and ovarian cancer syndrome.

View all

General information

The SALSA MS-MLPA Probemix ME053-A1 BRCA1-BRCA2-RAD51C is a research use only (RUO) assay for the detection of methylation status of one or more sequences of the BRCA1, BRCA2 and RAD51C genes.

BRCA1, BRCA2 and RAD51C are tumour suppressor genes that participate in DNA repair processes and genome integrity maintenance. Germline deleterious variants in these genes have been extensively described in hereditary breast and ovarian cancer patients, contributing to increased risks of developing the disease. Previous studies have also identified aberrant promoter methylation levels in BRCA1 and RAD51C genes, proposing a new mechanism of gene inactivation in early-onset breast and/or ovarian cancer (Hansmann et al. 2012, Evans et al. 2018). Furthermore, somatic methylation of BRCA1 and BRCA2 may result in reduced gene expression levels and contribute to tumorigenesis and development of sporadic breast/ovarian cancer (Esteller et al. 2000, Bosviel et al. 2011, Stordal et al. 2013). Moreover, promoter hypermethylation of BRCA1, BRCA2 and RAD51C genes has been described in triple-negative breast cancer and epithelial ovarian carcinomas, and it can guide effective therapeutic interventions with PARP inhibitors and serve as markers of therapy response (Veeck et al. 2010, Cunningham et al. 2014, Pennington et al. 2014, Sahnane et al. 2020, Menghi et al. 2022).

Regulatory status

SALSA MLPA Probemix ME053 BRCA1-BRCA2-RAD51C is for research use only (RUO) in all territories.

Product documentation

Version A1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
ME053-025R
SALSA MLPA Probemix ME053 BRCA1-BRCA2-RAD51C – 25 rxn
MS-MLPA
€ 296.00
ME053-050R
SALSA MLPA Probemix ME053 BRCA1-BRCA2-RAD51C – 50 rxn
MS-MLPA
€ 580.00
ME053-100R
SALSA MLPA Probemix ME053 BRCA1-BRCA2-RAD51C – 100 rxn
MS-MLPA
€ 1135.00

Required reagents

A general SALSA MLPA Reagent Kit and SALSA HhaI are required for MS-MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00
SMR50
SALSA HhaI – 115 μl
MS-MLPA
€ 48.00

Other products

These optional accessories can be ordered separately.

Item no.
Description
Technology
Price
SMR05
SALSA FFPE Solution (SFS) – 50 rxn (10 ml)
MLPA MS-MLPA
€ 47.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

The commercially available positive samples below can be used with the current (A1) version of this product.

  • DSMZ ACC-9 (U-266): Heterozygous deletion affecting the probes for ZMYM2, CENPJ, BRCA2, N4BP2L and PCCA5 on chromosome 13, and ATP1B2 and AKAP10 on chromosome 17. Gain affecting the probes for SGCA, COL1A1 and RAD51C on chromosome 17.
  • DSMZ ACC-10 (K-562): Heterozygous deletion affecting the probes for ZMYM2, CENPJ, BRCA2, N4BP2L and PCCA5 on chromosome 13.
  • DSMZ ACC-20 (BV-173), DSMZ ACC-78 (DAUDI): RAD51C on chromosome 17 is methylated.
  • DSMZ ACC-255 (CADO-ES1): Gain affecting the probes for SGCA, COL1A1 and RAD51C on chromosome 17.
  • DSMZ ACC-573 (SU-DHL-8): Gain affecting the probes for BRCA2 on chromosome 13. RAD51C on chromosome 17 is methylated.
  • DSMZ ACC-581 (HCT-116): Gain affecting the probes for SGCA, COL1A1 and RAD51C on chromosome 17.
  • Zymo Research Human Methylated & Non-methylated DNA Set (cat. no. D5014): Gain affecting the probes for SGCA, COL1A1 and RAD51C on chromosome 17. Non-Methylated DNA: Negative control - all targets are non-methylated. Methylated DNA: Positive control - all targets are methylated. Digestion control probe at 132 nt is not digested in this methylated control sample. The HhaI digestion of this probe is NOT dependent on the methylation state of the target DNA, as the GCGC site is located in the probe’s stuffer sequence.
  • Promega Methylated Human Control DNA Catalog #1231: Positive control - all targets are methylated. Digestion control probes at 198 and 211 nt are fully digested in this methylated control sample. The HhaI digestion of these probes is NOT dependent on the methylation state of the target DNA, as the GCGC site is located in the probe’s stuffer sequence.

Resources

General MLPA resources

MLPA education

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