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ME001 Tumour suppressor mix

SALSA MLPA ME001 Tumour suppressor mix detects copy number variations and methylation status of 25 tumour suppressor genes.

Specifications

Contents: 45 MLPA probes, including 28 probes for tumour suppressor genes, of which all provide information on the methylation status.

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on cancer.

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

ME011 Mismatch Repair Genes

Contains (MS)-MLPA probes for promoter regions of MLH1, MSH2, MSH6, PMS2 and EPCAM genes, and one BRAF V600E mutation-specific probe.

ME012 MGMT-IDH-TERT

Contains MS-MLPA probes for the MGMT promoter region as well as mutation-specific probes to detect the presence of point mutations in the IDH1, IDH2 genes and the TERT promoter.

ME024 9p21 CDKN2A/2B region

Contains (MS)-MLPA probes for CDKN2A/2B gene region, MIR31, MTAP and PAX5 genes.

View all

This product is for basic research and intended for experienced users only! Interpretation of results may be complicated, and MRC Holland may only be able to provide basic support.

General information

The SALSA MS-MLPA Probemix ME001 Tumour suppressor mix is a research use only (RUO) assay for the detection of aberrant methylation of one or more sequences of 25 tumour suppressor genes (TSG). This probemix can also be used to detect deletions/duplications in these TSGs in a DNA sample.

CpG-islands are located in or near the promoter region or other regulatory regions of approximately 50% of human genes. Aberrant methylation of CpG-islands has been shown to be associated with transcriptional inactivation of genes in a wide spectrum of human cancers. These TSGs are frequently silenced by methylation in tumours, but are unmethylated in blood-derived DNA. In addition, DNA methylation analysis can indicate in some cases from which type of tissue the tumour was derived.

Regulatory status

SALSA MLPA Probemix ME001 Tumour suppressor mix is for research use only (RUO) in all territories.

Product documentation

Version D1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
ME001-025R
SALSA MLPA Probemix ME001 Tumour suppressor mix – 25 rxn
MS-MLPA
€ 296.00
ME001-050R
SALSA MLPA Probemix ME001 Tumour suppressor mix – 50 rxn
MS-MLPA
€ 580.00
ME001-100R
SALSA MLPA Probemix ME001 Tumour suppressor mix – 100 rxn
MS-MLPA
€ 1135.00

Required reagents

A general SALSA MLPA Reagent Kit and SALSA HhaI are required for MS-MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00
SMR50
SALSA HhaI – 115 μl
MS-MLPA
€ 48.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

See this support article for commercially available positive samples that can be used with this product.

Resources

General MLPA resources

MLPA education

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