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ME034 Multi-locus Imprinting

SALSA® MLPA® Probemix ME034 Multi-locus Imprinting detects copy number variations and the methylation status of one or more sequences in 14 different differentially methylated regions (DMRs) on 8 different chromosomes.

Specifications

Contents: 40 MLPA probes, including 3 probes each for the H19 and PEG3 regions, 2 probes each for the KCNQ1OT1, MEST, MEG3, MEG8, SNRPN, PLAGL1, GRB10, and ZNF597 regions, and 5 probes for the GNAS complex locus. Of these probes, all 27 provide information on the methylation status of all regions mentioned.

Tissue: human genomic DNA.

Application: research on multi-locus imprinting disturbances, and finding the parental origin of triploid pregnancies for research purposes.

For research use only (RUO). Not for use in diagnostics.

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Key related products

ME028 Prader-Willi/Angelman

Probes for the 15q11 imprinted region implicated in Prader-Willi and Angelman syndromes.

ME030 BWS/RSS

Probes for the 11p15 imprinted region implicated in Beckwith-Wiedemann & Russel-Silver syndromes.

ME031 GNAS

Probes for the 20q13 imprinted GNAS region.

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General information

The SALSA MLPA Probemix ME034 Multi-locus Imprinting is a research use only (RUO) assay for the detection of aberrant methylation of one or more sequences in 14 different differentially methylated regions (DMRs) on eight different chromosomes. Applications include the study of multi-locus imprinting disturbances and finding the parental origin of triploid pregnancies. This probemix can also be used to detect deletions/duplications in the aforementioned chromosomal regions.

Genomic imprinting is the monoallelic expression of genes, dependent on the parental origin of the chromosome. It plays a role in growth and development. Imprinting disturbances originate from a disturbance in this monoallelic expression by disruption or epimutation of imprinted genes (Ishida et al. 2013).

Multi-locus imprinting disturbances (MLIDs) have been identified in several patients. For example, a considerable number of patients with 11p15-associated imprinting disturbances have been reported to also carry methylation changes at other chromosomal locations (Eggermann et al. 2014).

Triploidy is one of the most common chromosomal abnormalities, occurring in 1–2% of all human conceptuses. Triploid pregnancies can have either the chromosomal constitution of two maternal and one paternal set (digyny) or two paternal and one maternal set (diandry). The distinction between digyny and diandry is clinically important because the risk of the conceptus being molar and thereby inducing a risk for maternal complications (such as pre-eclampsia, postpartum haemorrhage and persistent gestational trophoblastic disease) is determined by the parental composition of the genome. As shown by Joergensen et al. (2013 and 2014), methylation-specific MLPA (MS-MLPA) analysis of imprinted regions is a reliable method to distinguish triploidies with a double paternal contribution from triploidies with a double maternal contribution.

Regulatory status

SALSA MLPA Probemix ME034 Multi-locus Imprinting is for research use only (RUO) in all territories.

Product documentation

Version D1

Version C1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
ME034-025R
SALSA MLPA Probemix ME034 Multi-locus Imprinting – 25 rxn
MS-MLPA
€ 286.00
ME034-050R
SALSA MLPA Probemix ME034 Multi-locus Imprinting – 50 rxn
MS-MLPA
€ 560.00
ME034-100R
SALSA MLPA Probemix ME034 Multi-locus Imprinting – 100 rxn
MS-MLPA
€ 1096.00

Required reagents

A general SALSA MLPA Reagent Kit and SALSA HhaI are required for MS-MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 348.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 348.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6152.00
SMR50
SALSA HhaI – 115 μl
MS-MLPA
€ 46.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

We have no information about specific commercially available positive samples that can be used with this product.

Resources

General MLPA resources

MLPA education

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