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P483 HER gene family

SALSA MLPA Probemix P483 HER gene family detects copy number variations in the EGFR (ERBB1/HER1), ERBB2 (HER2), ERBB3 (HER3) and ERBB4 (HER4) genes.

Specifications

Contents: 54 MLPA probes, including 9 probes for EGFR, 8 probes for ERBB2, 4 probes for ERBB3, and 5 probes for ERRB4.

Tissue: human genomic DNA, including DNA from fresh-frozen or FFPE tissue.

Application: research on cancer.

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

P078 Breast tumour

Contains probes for EGFR and ERBB2.

P105 Glioma

Contains probes for EGFR.

P175 Tumour Gain

Contains probes for EGFR and ERBB2.

View all

General information

The SALSA MLPA Probemix P483 HER gene family is a research use only (RUO) assay for the detection of deletions or duplications in the HER family of receptor tyrosine kinases. This gene family is comprised of four members promoting cell proliferation, motility and invasion via signalling through homo- or heterodimerization: EGFR (ERBB1/HER1), ERBB2 (HER2), ERBB3 (HER3), and ERBB4 (HER4) (Sergina and Moasser, 2007).

Amplifications in the Human Epidermal Growth Factor Receptor (HER) gene family are associated with a high invasiveness of human cancers, most notably in breast cancer (Gan et al, 2013; Sergina and Moasser, 2007). As transmembrane proteins, the products of these genes are suitable targets for anticancer drugs. For example, breast cancer patients with tumours overexpressing Her2 (20-30% of breast tumours; Mitri et al. 2012) are treated with trastuzumab (Herceptin), which impairs receptor signalling by binding to its extracellular domain.

EGFR amplifications have been observed in a variety of cancers, including glioblastomas and colorectal carcinoma (Ayati et al. 2020). These amplifications are sometimes paired with rearrangements, such as EGFRvIII (deletion of exons 2-7; Gan et al. 2013) rendering the receptor constitutively active.

Regulatory status

SALSA MLPA Probemix P483 HER gene family is for research use only (RUO) in all territories.

Product documentation

Version A1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P483-025R
SALSA MLPA Probemix P483 HER gene family – 25 rxn
MLPA
€ 296.00
P483-050R
SALSA MLPA Probemix P483 HER gene family – 50 rxn
MLPA
€ 580.00
P483-100R
SALSA MLPA Probemix P483 HER gene family – 100 rxn
MLPA
€ 1135.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

See this support article for commercially available positive samples that can be used with this product.

Resources

General MLPA resources

MLPA education

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