General information
The SALSA MLPA
Probemix P078 Breast tumour is a research use only (RUO) assay for the detection of deletions or duplications in 6
(ESR1), 7
(EGFR), 8p
(ZNF703, FGFR1, ADAM9, IKBKB), 8q
(PRDM14, MTDH, MYC), 11q
(CCND1, EMSY), 16q
(CDH1), 17q
(CPD, MED1, ERBB2, CDC6, TOP2A, MAPT, PPM1D, BIRC5), 19q
(CCNE1) and 20q
(AURKA). See Table 1 and Table 2 in the Product Description for more detailed information about all included chromosomal regions and genes.
This product is not CE/FDA registered for use in diagnostic procedures. The SALSA MLPA technique is covered by US patent 6,955,901 and corresponding patents outside the US. The purchase of this product includes a license to use only this amount of product solely for the purchaser’s own use.
Probemix content
P078-D3 Breast tumour contains 56 MLPA probes with amplification products between 127 and 500 nt. This includes 42 probes for the following chromosomal regions/genes: 6q
(ESR1), 7p
(EGFR),8p
(ZNF703, FGFR1, ADAM9, IKBKB), 8q
(PRDM14, MTDH, MYC), 11q
(CCND1, EMSY), 16q
(CDH1), 17q
(CPD,MED1, ERBB2, CDC6, TOP2A, MAPT, PPM1D, BIRC5), 19q12
(CCNE1) and 20q
(AURKA). In addition, 14 reference probes are included which target relatively copy number stable regions in various tumour types, including breast cancer. The identity of the genes detected by the reference probes is available in Table 3 of the Product Description. Partial probe sequences are available online (
www.mrcholland.com).
This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at
www.mrcholland.com.