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P078 Breast tumour

SALSA MLPA Probemix P078 Breast tumour detects copy number variations in genes and chromosomal regions associated with breast cancer.

Specifications

Contents: 56 MLPA probes, including 42 probes for the following regions and genes: 6q (ESR1), 7p (EGFR), 8p (ZNF703, FGFR1, ADAM9, IKBKB), 8q (PRDM14, MTDH, MYC), 11q (CCND1, EMSY), 16q (CDH1), 17q (CPD, MED1, ERBB2, CDC6, TOP2A, MAPT, PPM1D, BIRC5), 19q12 (CCNE1), and 20q (AURKA).

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on breast cancer.

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

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Contains probes for all exons of the BRCA1 gene.

P045 BRCA2/CHEK2

Contains probes for all exons of the BRCA2 gene and three probes for CHEK2, including one mutation-specific probe.

P087 BRCA1 Confirmation

Contains probes for all BRCA1 exons.

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General information

SALSA MLPA Probemix P078 Breast tumour is a research use only (RUO) assay for the detection of deletions or amplifications in genes/chromosomal regions of diagnostic/clinical importance in breast cancer, including 6q (ESR1), 7p (EGFR), 8p (ZNF703, FGFR1, ADAM9, IKBKB), 8q (PRDM14, MTDH, MYC), 11q (CCND1, EMSY), 16q (CDH1), 17q (CPD, MED1, ERBB2, CDC6, TOP2A, MAPT, PPM1D, BIRC5), 19q (CCNE1) and 20q (AURKA). See Table 1 and Table 2 in the product description for more detailed information about all included chromosomal regions and genes.

Regulatory status

SALSA MLPA Probemix P078 Breast tumour is for research use only (RUO) in all territories.

Product documentation

Version D3

Version D2

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P078-025R
SALSA MLPA Probemix P078 Breast tumour – 25 rxn
MLPA
€ 296.00
P078-050R
SALSA MLPA Probemix P078 Breast tumour – 50 rxn
MLPA
€ 580.00
P078-100R
SALSA MLPA Probemix P078 Breast tumour – 100 rxn
MLPA
€ 1135.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

The commercially available positive samples below can be used with the current (D3) version of this product.

Germline samples

  • Coriell NA00959: Heterozygous duplication affecting the probes for CCND1 and EMSY on chromosome 11.
  • Coriell NA02030: Heterozygous duplication affecting the probes for ZNF703, FGFR1, ADAM9, IKBKB, PRDM14, MTDH and MYC on chromosome 8.
  • Coriell NA03999: Heterozygous deletion affecting the probes for MYC on chromosome 8.
  • Coriell NA07081: Heterozygous duplication affecting the probes for EGFR on chromosome 7.
  • Coriell NA07994: Heterozygous duplication affecting the probes for ESR1 on chromosome 6.
  • Coriell NA08123: Heterozygous duplication affecting the probes for AURKA on chromosome 20.
  • Coriell NA12074: Heterozygous deletion affecting the probes for CDH1 on chromosome 16.
  • Coriell NA14485: Heterozygous duplication affecting the probes for ZNF703, FGFR1, ADAM9 and IKBKB on chromosome 8.
  • Coriell NA16445: Heterozygous duplication affecting the probes for BIRC5 on chromosome 17.

Cancer cell line samples

Some of the reference probes are also affected by CNAs.

  • DSMZ ACC-200 (COLO-824): Gain affecting the probes for EGFR on chromosome 7. Gain affecting the probes for PRDM14, MTDH and MYC on chromosome 8. Gain affecting the probes for AURKA on chromosome 20.
  • DSMZ ACC-432 (8-MG-BA): Gain affecting the probes for EGFR on chromosome 7. Gain affecting the probes for PRDM14, MTDH and MYC on chromosome 8. Gain affecting the probes for CCND1 and EMSY on chromosome 11. Gain affecting the probes for MAPT, PPM1D and BIRC5 on chromosome 17. Gain affecting the probes for CCNE1 on chromosome 19. Gain affecting the probes for AURKA on chromosome 20.
  • DSMZ ACC-460 (CAL-148): Gain affecting the probes for EGFR on chromosome 7. Heterozygous deletion affecting the probes for CDH1 on chromosome 16. Gain affecting the probes for AURKA on chromosome 20.

Resources

General MLPA resources

MLPA education

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