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SALSA MLPA Probemix P383 T-ALL

T-lineage acute lymphoblastic leukaemia (T-ALL)

Region: various

General information
The SALSA MLPA Probemix P383 T-ALL is a research use only (RUO) assay for the detection of deletions or duplications in 13 different chromosomal regions, which are suggested to be of diagnostic and/or prognostic importance in T-ALL.

T-lineage acute lymphoblastic leukemia (T-ALL) is a clonal malignant disorder of immature T-cells, which accounts for about 15% of paediatric and 25% of adult ALL cases (Iacobucci and Mullighan 2017). Although prognosis has improved due to effective treatment protocols, around 25% of paediatric and 50-70% of adult patients still experience relapse. T-ALL is genetically heterogeneous, and comprises multiple distinct subtypes defined by chromosomal rearrangements that usually involve one of the T-cell receptor (TCR) loci, but non-TCR-driven translocations are also detected. In addition, genome-wide characterisation has identified several novel amplifications, deletions and point mutations in T-ALL samples that are of interest to better define T-ALL subgroups (Girardi et al. 2017).

This SALSA MLPA probemix is not CE/FDA registered for use in diagnostic procedures. Purchase of this product includes a limited license for research purposes.

Probemix content
The SALSA MLPA Probemix P383-A2 T-ALL contains 56 MLPA probes with amplification products between 130 and 504 nucleotides (nt). This includes probe(s) for 13 chromosomal regions, including STIL-TAL1 (1p33), LEF1 (4q25), CASP8AP2 (6q15), MYB (6q23), EZH2 (7q36) MLLT3+MTAP+CDKN2A/B (9p21), NUP214-ABL1 (9q34), PTEN (10q23), LMO1 (11p15), LMO2 (11p13), NF1+SUZ12 (17q11), PTPN2 (18p11) and PHF6 (Xq26). In this probemix, 40 out of the 56 MLPA probes are used as reference probes, as they are spread over a number of different chromosomal regions and it is expected that the majority of these probes will have a normal copy number in most samples. Complete probe sequences are available online (www.mrcholland.com).

This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mrcholland.com.

Order Items

Probemix

Item no.
Description
Technology
Price
P383-025R
SALSA MLPA Probemix P383 T-ALL – 25 rxn
€ 243.00
P383-050R
SALSA MLPA Probemix P383 T-ALL – 50 rxn
€ 486.00
P383-100R
SALSA MLPA Probemix P383 T-ALL – 100 rxn
€ 972.00

Required Reagents

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA EK1 reagent kit – 100 rxn – FAM
€ 300.00
EK1-Cy5
SALSA MLPA EK1 reagent kit – 100 rxn – Cy5
€ 300.00
EK5-FAM
SALSA MLPA EK5 reagent kit – 500 rxn – FAM
€ 1380.00
EK5-Cy5
SALSA MLPA EK5 reagent kit – 500 rxn – Cy5
€ 1380.00
EK20-FAM
SALSA MLPA EK20 reagent kit – 2000 rxn – FAM
€ 5295.00

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Improvements

One IL3RA probe and one flanking probe have been removed, and one flanking probe replaced.