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P118 WT1

SALSA MLPA Probemix P118 WT1 detects copy number variations in the WT1 region and the AMER1 gene.

Specifications

Contents: 34 MLPA probes, including 11 probes for the WT1 region (covering all WT1 exons), and 3 probes for AMER1.

Tissue: human genomic DNA.

Application: research on Wilms' tumour.

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

P380 Wilms' tumour

Targets various genes and regions associated with Wilms' tumour, including WT1 and AMER1.

P219 PAX6

Targets the 11p13-14 region, which includes WT1, and SOX2, associated with hereditary ocular malformations.

ME030 BWS/RSS

Constitutional 11p15 abnormalities have been identified in 3% of non-syndromic Wilms' tumour cases with the use of ME030.

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General information

The SALSA MLPA Probemix P118 WT1 is a research use only (RUO) assay for the detection of deletions or duplications in the WT1 gene region and the AMER1 gene, which are associated with Wilms tumours. The majority of Wilms tumour, or nephroblastoma, is caused by defects in the WT1 gene on chromosome 11p13. The WT1 gene product is required for normal formation of the genitourinary system and mesothelial tissues. Mutations in WT1 gene have also been observed in patients with Denys-Drash syndrome, Frasier syndrome, Meacham syndrome, and type 4 nephrotic syndrome. Mutations in the WT1 gene are observed in only 5% of the sporadic Wilms tumours. However, more than 90% of patients with Denys-Drash syndrome, which includes Wilms tumour, carry constitutional intragenic WT1 mutations. Large chromosomal deletions including the WT1 gene cause WAGR syndrome. We recommend the P219 PAX6 probemix to study the extent of chromosomal deletions in WAGR syndrome patients. Deletions at chromosome Xq11 have been implicated in sporadic Wilms tumours. The only gene in the interval is AMER1, which is also called WTX for 'Wilms tumour gene on the X chromosome'.

More information is available at https://www.ncbi.nlm.nih.gov/books/NBK1294/

Regulatory status

SALSA MLPA Probemix P118 WT1 is for research use only (RUO) in all territories.

Product documentation

Version C3

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P118-025R
SALSA MLPA Probemix P118 WT1 – 25 rxn
MLPA
€ 286.00
P118-050R
SALSA MLPA Probemix P118 WT1 – 50 rxn
MLPA
€ 560.00
P118-100R
SALSA MLPA Probemix P118 WT1 – 100 rxn
MLPA
€ 1096.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 348.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 348.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6152.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

See this support article for commercially available positive samples that can be used with this product.

Resources

General MLPA resources

MLPA education

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