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P303 Medulloblastoma mix 3

SALSA MLPA Probemix P303 Medulloblastoma mix 3 detects copy number variations in several regions on chromosomes 1, 4q, 5q, 8, 10 and 20 that may be related to medulloblastoma.

Specifications

Contents: 43 MLPA probes, including 31 probes for chromosomes 1, 4q, 5q, 8, 10 and 20.

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on medulloblastoma (MB).

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

P251 NB mix 1

Contains probes for multiple chromosomal regions that frequently show copy number changes in neuroblastoma tumours (chromosomes 1, 3 and 11).

P252 NB mix 2

Contains probes for multiple chromosomal regions that frequently show copy number changes in neuroblastoma tumours (chromosomes 2 (MYCN region) and 17).

P253 NB mix 3

Contains probes for multiple chromosomal regions that frequently show copy number changes in neuroblastoma tumours (chromosomes 4, 7, 9, 12 and 14).

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General information

SALSA MLPA Probemix P303 Medulloblastoma mix 3 is a research use only (RUO) assay for the detection of deletions or gains in chromosomes 1, 4q, 5q, 8, 10 and 20, which are suggested to be associated with medulloblastoma.

Medulloblastoma (MB) is the most common paediatric primary central nervous system (CNS) cancer type, accounting for 15% to 20% of CNS tumours in patients under the age of 20. It is a highly invasive embryonal neuroepithelial tumour that arises in the cerebellum and has a tendency to disseminate throughout the CNS early in its course. Overall survival is 50-60% at five years, although this decreases to 30% in the longer term due to local recurrence and/or metastasis. There are four distinct molecular subtypes of MB (WNT, sonic hedgehog (SHH), Group 3, and Group 4) which can be used for patient risk stratification and that have the potential to guide new therapeutic strategies for the treatment of MB (Taylor et al. 2012). These molecular subtypes of MB include characteristic and recurrent copy number alterations, which are covered by the P301, P302 and P303 Medulloblastoma probemixes.

Regulatory status

SALSA MLPA Probemix P303 Medulloblastoma mix 3 is for research use only (RUO) in all territories.

Product documentation

Version A3

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P303-025R
SALSA MLPA Probemix P303 Medulloblastoma mix 3 – 25 rxn
MLPA
€ 296.00
P303-050R
SALSA MLPA Probemix P303 Medulloblastoma mix 3 – 50 rxn
MLPA
€ 580.00
P303-100R
SALSA MLPA Probemix P303 Medulloblastoma mix 3 – 100 rxn
MLPA
€ 1135.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

The commercially available positive samples below can be used with the current (A3) version of this product.

Chromosome 1

  • Coriell NA00214: Heterozygous deletion affecting the probe for KIF14.
  • Coriell NA05347: Heterozygous duplication affecting the probes for LIN9 and SH3BP5L.
  • Coriell NA17941: Heterozygous duplication affecting the probes for KIF14, LIN9 and SH3BP5L.
  • Coriell NA22995: Heterozygous deletion affecting the probe for TP73.

Chromosome 4

Chromosome 5

Chromosome 8

  • Coriell NA02030: Heterozygous duplication affecting the probes for PINX1, GNHR1, FGFR1, CHD7, MYC and PTP4A3.
  • Coriell NA03999: Heterozygous deletion affecting the probe for MYC.
  • Coriell NA10932: Heterozygous deletion affecting the probe for PINX1.
  • Coriell NA14485: Heterozygous duplication affecting the probes for GNRH and FGFR1.
  • Coriell NA20263: Heterozygous duplication affecting the probe for PTP4A3.

Chromosome 10

  • Coriell NA00959: Heterozygous duplication affecting the probes for SUFU, MXI1 and DMBT1.
  • Coriell NA05299: Heterozygous deletion affecting the probe for DMBT1.
  • Coriell NA06936: Heterozygous deletion affecting the probe for KLF6.
  • Coriell NA20125: Heterozygous duplication affecting the probes for PTEN, SUFU, MXI1 and DMBT1.
  • Coriell NA21877: Heterozygous deletion affecting the probe for NEBL.

Chromosome 20

Various

Resources

General MLPA resources

MLPA education

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