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P301 Medulloblastoma mix 1

SALSA MLPA Probemix P301 Medulloblastoma mix 1 detects copy number variations in several regions on chromosomes 6, 14q, 16 and 17 that may be related to medulloblastoma.

Specifications

Contents: 47 MLPA probes, including 35 probes for chromosomes 6, 14q, 16 and 17.

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on medulloblastoma (MB).

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

P251 NB mix 1

Contains probes for multiple chromosomal regions that frequently show copy number changes in neuroblastoma tumours (chromosomes 1, 3 and 11).

P252 NB mix 2

Contains probes for multiple chromosomal regions that frequently show copy number changes in neuroblastoma tumours (chromosomes 2 (MYCN region) and 17).

P253 NB mix 3

Contains probes for multiple chromosomal regions that frequently show copy number changes in neuroblastoma tumours (hromosomes 4, 7, 9, 12 and 14).

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This product is for basic research and intended for experienced users only! Interpretation of results may be complicated, and MRC Holland may only be able to provide basic support.

General information

The SALSA MLPA Probemix P301 Medulloblastoma mix 1 is a research use only (RUO) assay for the detection of deletions or gains in chromosomes 6, 14q, 16 and 17, which are suggested to be associated with medulloblastoma.

Medulloblastoma (MB) is the most common paediatric primary central nervous system (CNS) cancer type, accounting for 15% to 20% of CNS tumours in patients under the age of 20. It is a highly invasive embryonal neuroepithelial tumour that arises in the cerebellum and has a tendency to disseminate throughout the CNS early in its course. Overall survival is 50-60% at five years, although this decreases to 30% in the longer term due to local recurrence and/or metastasis. There are four distinct molecular subtypes of MB (WNT, sonic hedgehog (SHH), Group 3, and Group 4), which can be used for patient risk stratification and that have the potential to guide new therapeutic strategies for the treatment of MB (Taylor et al. 2012). These molecular subtypes of MB include characteristic and recurrent copy number alterations, which are covered by the P301, P302 and P303 Medulloblastoma probemixes.

Regulatory status

SALSA MLPA Probemix P301 Medulloblastoma mix 1 is for research use only (RUO) in all territories.

Product documentation

Version B1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P301-025R
SALSA MLPA Probemix P301 Medulloblastoma mix 1 – 25 rxn
MLPA
€ 296.00
P301-050R
SALSA MLPA Probemix P301 Medulloblastoma mix 1 – 50 rxn
MLPA
€ 580.00
P301-100R
SALSA MLPA Probemix P301 Medulloblastoma mix 1 – 100 rxn
MLPA
€ 1135.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

The commercially available positive samples below can be used with the current (B1) version of this product.

Chromosome 6

  • Coriell NA01221: Heterozygous duplication affecting the probe for MAP3K7.
  • Coriell NA12721: Heterozygous duplication affecting the probes for E2F3 and SOX4.
  • Coriell NA06802: Heterozygous deletion affecting the probe for MAP3K4.
  • Coriell NA07994: Heterozygous duplication affecting the probes for MYB, MYCT1 and MAP3K4.
  • Coriell NA09367: Heterozygous duplication affecting the probes for SGK1 and MYB.

Chromosome 16

  • Coriell NA02325: Heterozygous duplication affecting the probes for AXIN1 and MEFV.
  • Coriell NA05875: Heterozygous deletion affecting the probe for TGFB1I1.
  • Coriell NA05966: Heterozygous duplication affecting the probes for OTX2 and MLH3
  • Coriell NA08039: Heterozygous duplication affecting the probe for MEFV.
  • Coriell NA09687: Heterozygous deletion affecting the probe for AXIN1. Heterozygous duplication affecting the probes for ZFHX3 and FANCA.
  • Coriell NA12074: Heterozygous deletion affecting the probe for ZFHX3.
  • Coriell NA13284: Heterozygous duplication affecting the probe for AXIN1.

Chromosome 17

  • Coriell NA06047: Heterozygous deletion affecting the probes for HIC1, PAFAH1B1 and ATP2A3.
  • Coriell NA13476: Heterozygous deletion affecting the probe for PRPSAP2.
  • Coriell NA16445: Heterozygous duplication affecting the probes for TK1, BIRC5, ARHGDIA and RAC3.

Various

  • DSMZ ACC-740 (HD-MB03): Heterozygous deletion affecting the probes for SGK1, MYB, MYCT1 and MAP3K4 on chromosome 6, and the probes for HIC1, PAFAH1B1, ATP2A3, TP53, RCVRN and PRPSAP2 on chromosome 17. Gain affecting the probe for OTX2 on chromosome 14, and the probes for ERBB2, RARA, TOP2A, RPS6KB1, PPM1D, AXIN2, TK1, BIRC5, ARHGDIA and RAC3 on chromosome 17. In our tests a high signal was observed for OTX, indicating amplification.

Resources

General MLPA resources

MLPA education

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