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SALSA MLPA Probemix P302 Medulloblastoma mix 2

Medulloblastoma

Region: Chromosomes 2, 3, 7 and 9

This product is for basic research and intended for experienced users only! Interpretation of results may be complicated, and MRC Holland may only be able to provide basic support.

General information: The SALSA MLPA Probemix P302 Medulloblastoma mix 2 is a research use only (RUO) assay for the detection of deletions or duplications in the chromosomes 2, 3, 7 and 9, which are thought to be associated with medulloblastoma.

Medulloblastoma (MB) is the most common paediatric primary central nervous system (CNS) tumour and accounts for between 15% and 20% of CNS tumours in patients under the age of 20. It is a highly invasive embryonal neuroepithelial tumour that arises in the cerebellum and has a tendency to disseminate throughout the CNS early in its course. Overall survival is 50-60% at five years, although this decreases to 30% in the longer term due to local recurrence and/or metastasis. There are four distinct molecular subtypes of MB (WNT, sonic hedgehog (SHH), Group 3, and Group 4) which can be used for patient risk stratification and that have the potential to identify new therapeutic strategies for the treatment of MB (Taylor et al. 2012). These molecular subtypes of MB include also characteristic and recurrent copy number alterations, which are covered by the P301, P302 and P303 Medulloblastoma probemixes.

This SALSA MLPA Probemix is not CE/FDA registered for use in diagnostic procedures. Purchase of this product includes a limited license for research purposes.

Probemix content: The SALSA MLPA Probemix P302-A3 Medulloblastoma mix 2 contains 50 MLPA probes with amplification products between 121 and 500 nucleotides (nt). This includes 37 probes for the chromosomes 2, 3, 7 and 9. In addition, 13 reference probes are included that target relatively copy number stable regions in various cancer types including medullobalstoma. Complete probe sequences and the identity of the genes detected by the reference probes are available in Table 2b and online (www.mlpa.com).

This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mlpa.com.

Order Items

Probemix

Item no.
Description
Technology
Price
P302-025R
SALSA MLPA Probemix P302 Medulloblastoma mix 2 – 25 rxn
€ 243.00
P302-050R
SALSA MLPA Probemix P302 Medulloblastoma mix 2 – 50 rxn
€ 486.00
P302-100R
SALSA MLPA Probemix P302 Medulloblastoma mix 2 – 100 rxn
€ 972.00

Required Reagents

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA EK1 reagent kit – 100 rxn – FAM
€ 300.00
EK1-Cy5
SALSA MLPA EK1 reagent kit – 100 rxn – Cy5
€ 300.00
EK5-FAM
SALSA MLPA EK5 reagent kit – 500 rxn – FAM
€ 1380.00
EK5-Cy5
SALSA MLPA EK5 reagent kit – 500 rxn – Cy5
€ 1380.00
EK20-FAM
SALSA MLPA EK20 reagent kit – 2000 rxn – FAM
€ 5295.00

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SALSA MLPA Probemix P252 NB mix 2

Contains probes for multiple chromosomal regions that frequently show copy number changes in neuroblastoma tumours (chromosomes 2 (MYCN region) and 17).

SALSA MLPA Probemix P253 NB mix 3

Contains probes for multiple chromosomal regions that frequently show copy number changes in neuroblastoma tumours (hromosomes 4, 7, 9, 12 and 14).

Improved Sep 16, 2020

SALSA MLPA Probemix P301 Medulloblastoma mix 1

Contains probes for chromosomes 6, 14q, 16 and 17.

SALSA MLPA Probemix P303 Medulloblastoma mix 3

Contains probes for chromosomes 1, 4q, 5q, 8, 10 and 20.

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Improvements

Several reference probes have been replaced and several probes have a change in length.