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P253 NB mix 3

SALSA MLPA Probemix P253 NB mix 3 detects copy number variations in several regions on chromosomes 4, 7, 9, 12 and 14 related to neuroblastoma.

Specifications

Contents: 47 MLPA probes, including 33 probes for chromosomes 4, 7, 9, 12 and 14.

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on neuroblastoma (NB).

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

ME024 9p21 CDKN2A/2B region

Contains probes for the detection of both copy number and methylation status of genes in the 9p21 (CDKN2A and CDKN2B) region.

P056 TP53

Contains at least one probe for each exon of TP53.

P088 Oligodendroglioma 1p-19q

Contains probes for the 1p and 19q chromosomal arms, CDKN2A & CDKN2B genes and IDH1 R132H/C and IDH2 R172M/K point mutations.

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General information

SALSA MLPA Probemixes P251/P252/P253 NB mix 1, 2 & 3 are research use only (RUO) assays for copy number determination of several chromosomal regions that frequently show copy number changes in neuroblastoma tumours.

Neuroblastoma (NB) is a relatively common paediatric cancer that usually occurs sporadically and frequently originates from one of the adrenal glands. NB is characterized by striking clinical heterogeneity, including cases that show spontaneous tumour regression. NB accounts up to 10% of all paediatric cancers. Several acquired genetic alterations such as amplification of the MYCN oncogene, deletions of chromosome bands 1p36 and 11q23 and unbalanced gains of 17q regions have been well-characterized and show correlation with tumour behaviour, including response to treatment. For review please see e.g. Ambros et al. (2009) and Ahmed et al. (2017).

Regulatory status

SALSA MLPA Probemix P253 NB mix 3 is for research use only (RUO) in all territories.

Product documentation

Version D2

Version D1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P253-025R
SALSA MLPA Probemix P253 NB mix 3 – 25 rxn
MLPA
€ 296.00
P253-050R
SALSA MLPA Probemix P253 NB mix 3 – 50 rxn
MLPA
€ 580.00
P253-100R
SALSA MLPA Probemix P253 NB mix 3 – 100 rxn
MLPA
€ 1135.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

The commercially available positive samples below can be used with the current (D2) version of this product.

Chromosome 4

  • Coriell NA00501: Heterozygous duplication affecting the probes for IL2, GLRB and KLKB1.
  • Coriell NA00782: Heterozygous duplication affecting the probes for GNRHR and IL2.
  • Coriell NA03013: Heterozygous deletion affecting the probes for GLRB and KLKB1.
  • Coriell NA03435: Heterozygous deletion affecting the probes for SPON2, WSF1 and KCNIP4.
  • Coriell NA10947: Heterozygous duplication affecting the probes for SPON2, WSF1 and KCNIP4.

Chromosome 7

Chromosome 9

  • Coriell NA02819: Heterozygous duplication affecting the probes for PTPRD, CDKN2A and DNAI1.
  • Coriell NA13685: Heterozygous duplication affecting the probes for POMT1 and TSC1.

Chromosome 12

  • Coriell NA08035: Heterozygous duplication affecting the probes for ERC1, CDKN1B and PKP2.

Chromosome 14

  • Coriell NA09888: Heterozygous deletion affecting the probe for ATL1.
  • Coriell NA10074: Heterozygous duplication affecting the probes for NFKBIA, ATL1, TGFB3 and MOAP1.

Various

  • Coriell NA10313: Heterozygous duplication affecting the probes for GLRB and KLKB1 on chromosome 4. Heterozygous deletion affecting the probe for SHH on chromosome 7.
  • Coriell NA14946: Heterozygous deletion affecting the probes for PTPRD on chromosome 9. Heterozygous duplication affecting the probe for ERC1 on chromosome 12.

Resources

General MLPA resources

MLPA education

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