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SALSA MLPA Probemix P182 Centromere mix 2

Centromeric testing

Region: Centromeres

General information: The SALSA MLPA Probemixes P181 Centromere mix 1 and P182 Centromere mix 2 are research use only (RUO) assays for the detection of deletions or duplications in genes close to the centromeres of all chromosomes, with the exception of the Y-chromosome. In most cases, probes are included for the first well-characterised gene in the centromeric region. Possible applications of these probemixes are in cancer research, as well as for characterisation of marker chromosomes.

Probemix content: The SALSA MLPA Probemix P181-B2 Centromere mix 1 contains 46 MLPA probes with amplification products between 130 and 450 nucleotides (nt). The SALSA MLPA Probemix P182-B2 Centromere mix 2 contains 46 MLPA probes with amplification products between 131 and 453 nt. Both probemixes include one probe for each of the chromosome arms (except the Y-chromosome). For the acrocentric chromosomes (13, 14, 15, 21 and 22), which have more than 10 Mb of repeat sequences at one end covering most or all of the p-arms, there are two probes on the q-arm in each probemix, close to the centromere. Probes in P181-B1 and P182-B2 detect different sequences. Complete probe sequences are available online (www.mlpa.com).

The P181-B2 Probemix contains ten quality control fragments generating amplification products between 64 and 121 nt: four DNA Quantity Fragments (Q-fragments), two DNA Denaturation Fragments (D-fragments), one Benchmark Fragment, one chromosome X and two chromosome Y-specific fragments.

The P182-B2 Probemix contains ten quality control fragments generating amplification products between 64 and 118 nt: four DNA Quantity Fragments (Q-fragments), two DNA Denaturation Fragments (D-fragments), one Benchmark Fragment, one chromosome X and two chromosome Y-specific fragments.

Order Items

Probemix

Item no.
Description
Technology
Price
P182-025R
SALSA MLPA Probemix P182 Centromere mix 2 – 25 rxn
€ 243.00
P182-050R
SALSA MLPA Probemix P182 Centromere mix 2 – 50 rxn
€ 486.00
P182-100R
SALSA MLPA Probemix P182 Centromere mix 2 – 100 rxn
€ 972.00

Required Reagents

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA EK1 reagent kit – 100 rxn – FAM
€ 300.00
EK1-Cy5
SALSA MLPA EK1 reagent kit – 100 rxn – Cy5
€ 300.00
EK5-FAM
SALSA MLPA EK5 reagent kit – 500 rxn – FAM
€ 1380.00
EK5-Cy5
SALSA MLPA EK5 reagent kit – 500 rxn – Cy5
€ 1380.00
EK20-FAM
SALSA MLPA EK20 reagent kit – 2000 rxn – FAM
€ 5295.00

Related Products

SALSA MLPA Probemix P181 Centromere mix 1

Centromere mix 1 is complementary to this probemix.

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