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SALSA MLPA Probemix P118 WT1

Wilms tumour; WAGR syndrome; Denys-Drash syndrome; Frasier syndrome; Meacham syndrome; Nephrotic syndrome type 4 (NPHS4)

Region: WT1 11p13; AMER1 Xq11.1

General Information: The SALSA MLPA Probemix P118 WT1 is a research use only (RUO) assay for the detection of deletions or duplications in the WT1 gene region and the AMER1 gene, which are associated with Wilms tumours. The majority of Wilms tumour, or nephroblastoma, is caused by defects in the WT1 gene on chromosome 11p13. The WT1 gene product is required for normal formation of the genitourinary system and mesothelial tissues. Mutations in WT1 gene have also been observed in patients with Denys-Drash syndrome, Frasier syndrome, Meacham syndrome, and type 4 nephrotic syndrome. Mutations in the WT1 gene are observed in only 5% of the sporadic Wilms tumours. However, more than 90% of patients with Denys-Drash syndrome, which includes Wilms tumour, carry constitutional intragenic WT1 mutations. Large chromosomal deletions including the WT1 gene cause WAGR syndrome. We recommend the P219 PAX6 probemix to study the extent of chromosomal deletions in WAGR syndrome patients. Deletions at chromosome Xq11 have been implicated in sporadic Wilms tumours. The only gene in the interval is AMER1, which is also called WTX for 'Wilms tumour gene on the X chromosome'.

More information is available at https://www.ncbi.nlm.nih.gov/books/NBK1294/

This SALSA MLPA Probemix is not CE/FDA registered for use in diagnostic procedures. Purchase of this product includes a limited license for research purposes.

Probemix content: The SALSA MLPA Probemix P118-C3 WT1 contains 34 MLPA probes with amplification products between 130 and 409 nucleotides (nt). This includes 17 probes for the WT1 gene and surrounding region. Furthermore, it also contains three probes for the AMER1 gene. In addition, 12 reference probes are included and detect 12 different autosomal chromosomal locations. Complete probe sequences and the identity of the genes detected by the reference probes is available online (www.mlpa.com).

This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity Fragments (Q-fragments), two DNA Denaturation Fragments (D-fragments), one benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mlpa.com.

Order Items

Probemix

Item no.
Description
Technology
Price
P118-025R
SALSA MLPA Probemix P118 WT1 – 25 rxn
€ 281.00
P118-050R
SALSA MLPA Probemix P118 WT1 – 50 rxn
€ 550.00
P118-100R
SALSA MLPA Probemix P118 WT1 – 100 rxn
€ 1075.00

Required Reagents (Sold Separately)

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM
€ 341.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5
€ 341.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM
€ 1571.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5
€ 1571.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM
€ 6037.00

Related Products

SALSA MLPA Probemix ME030 BWS/RSS

Beckwith-Wiedemann syndrome. Constitutional 11p15 abnormalities have been identified in 3% of non-syndromic Wilms tumour cases with the use of this ME030 MS-MLPA probemix.

SALSA MLPA Probemix P219 PAX6

Contains probes for PAX6, WT1, and WAGR region on 11p13.

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