General information
The SALSA MLPA Probemix P294 Tumour Loss is a research use only (RUO) assay for the detection of copy number aberrations in 15 chromosomal regions that are frequently deleted or mutated in tumour samples.

This SALSA MLPA probemix is not CE/FDA registered for use in diagnostic procedures. Purchase of this product includes a limited license for research purposes.

Probemix content
The SALSA MLPA Probemix P294-C1 Tumour Loss contains 63 MLPA probes with amplification products between 121 and 504 nucleotides (nt). This includes at least 2 probes for the following regions or genes: 1p36, 13q14 (RB1), AMER1, APC, BRCA1/2, CDKN2A/2B, FHIT, FKBP8, NF1, PTCH1, PTEN, SMAD4, SMARCB1, STK11, TP53, TSC1/2, VHL and WT1. In addition, 12 reference probes are included and detect different autosomal chromosomal locations that are relatively copy number stable in most cancer types. Complete probe sequences and the identity of the genes detected by the reference probes are available online (www.mrcholland.com).

This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mrcholland.com.