General information: The SALSA MLPA Probemix P294 Tumour Loss is a research use only (RUO) assay for the detection of copy number aberrations in 15 chromosomal regions, which are frequently deleted in tumour samples.

Probemix content: The SALSA MLPA Probemix P294-C1 Tumour Loss contains 63 MLPA probes with amplification products between 121 and 503 nucleotides (nt). This includes at least two probes for the following regions or genes: 1p36, 13q14 (RB1), AMER1, APC, BRCA1/2, CDKN2A/B, FHIT, FKBP8, NF1, PTCH1, PTEN, SMAD4, SMARCB1, STK11, TP53, TSC1/2, VHL, and WT1. In addition, 12 reference probes are included and detect different autosomal chromosomal locations which are relatively quiet in most tumour types. However, it should be noted that tumour karyotypes can harbour multiple numerical and structural aberrations, which can complicate interpretation of these reference probes. Complete probe sequences and the identity of the genes detected by the reference probes is available in Table 2b and online (www.mlpa.com).

This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment (see table below). More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mlpa.com.