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P283 TPMT

SALSA MLPA Probemix P283 TPMT detects copy number variations in the TPMT gene.

Specifications

Contents: 22 MLPA probes, including 10 probes for TPMT of which 1 detects the wild-type sequence of the c.719A>G mutation, and probes for TPMT c.460G>A and c.238G>C.

Tissue: human genomic DNA.

Application: research on thiopurine S-methyltransferase (TPMT) deficiency.

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

P103 DPYD

Contains probes for the DPYD gene.

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General information

SALSA MLPA Probemix P283 TPMT is a research use only (RUO) assay for the detection of deletions or duplications in the TPMT gene, which is associated with thiopurine S-methyltransferase deficiency (TPMT deficiency). This probemix can also be used to detect the presence of the c.460G>A/p.A154T (TPMT*3B allele), c.719A>G/p.Y240C (TPMT*3C allele), or both (TPMT*3A allele), and the c.238G>C/p.A80P (TPMT*2 allele) point mutations.

Defects in the thiopurine S-methyltransferase (TPMT) gene are the cause of TPMT deficiency. TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosuppressant or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukaemia, autoimmune disease, and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus.

Regulatory status

SALSA MLPA Probemix P283 TPMT is for research use only (RUO) in all territories.

SALSA Sample DNA for this product

SALSA Binning DNA SD087 is an artificial DNA sample with a signal for all probes in the P283 TPMT probemix. Inclusion of a reaction with SD087 in initial experiments and in experiments following a change in electrophoresis conditions is recommended to aid in the creation of a bin set that links peaks to the probes that produce them. Binning DNA cannot be used as a reference sample in the MLPA data analysis, and cannot be used to quantify the signals of mutation-specific probes.

A vial of SALSA Binning DNA SD087 is included with every order of the P283 TPMT probemix, but it is possible to order additional vials separately.

For more information, see the product description.

Product documentation

Version B2

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P283-025R
SALSA MLPA Probemix P283 TPMT – 25 rxn
MLPA
€ 286.00
P283-050R
SALSA MLPA Probemix P283 TPMT – 50 rxn
MLPA
€ 560.00
P283-100R
SALSA MLPA Probemix P283 TPMT – 100 rxn
MLPA
€ 1096.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 348.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 348.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6152.00

Sample DNAs (included)

A vial is included with every order of this probemix, but additional vials can also be purchased separately.

Item no.
Description
Technology
Price
SD087
SALSA Binning DNA SD087 – 6 rxn
MLPA
€ 24.15

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

We have no information about specific commercially available positive samples that can be used with this product.

Resources

General MLPA resources

MLPA education

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