Choose your country to see the products for your location

P200 Reference-1

SALSA MLPA Probemix P200 Reference-1 contains reference probes and control fragments to which 'home-made' synthetic MLPA or MS-MLPA probes can be added.

Specifications

Contents: 10 MLPA reference probes with autosomal targets, and 8 control fragments.

Tissue: human genomic DNA.

Application: reference and control probe set as basis for a 'home-made' probemix.

For research use only (RUO). Not for use in diagnostics.

Quick links

List Prices Documentation

Key related products

P300 Reference-2

Alternative basis for a 'home-made' probemix with reference probes distributed over the size range of the probemix and with more control fragments. P300 Reference-2 is recommended when a lower number of synthetic probes is added.

View all

We are no longer able to offer support on the design of synthetic MLPA probes. This probemix will continue to be available for existing users.

General information

The SALSA MLPA Probemix P200 Reference-1 is a research use only (RUO) assay which contains reference probes and control fragments specific for unique human DNA sequences. The P200 probemix offers a collection of thoroughly-tested MLPA reference probes and control fragments to which 'home-made' synthetic (MS-)MLPA probes can be added. Not only do these control fragments and reference probes provide extra assurance about the quality of home-made probes and of the MLPA reaction performed, they also facilitate the data analysis of your synthetic probemix and maximise the number of synthetic probes you can include.

Next to the P200, MRC Holland also offers the P300 Reference-2 probemix. While the P200 leaves the entire range from 82-173 nt open for the inclusion of synthetic probes, the P300 probemix has reference probes distributed over the whole size range of the probemix. This is especially advantageous for correction of the signal sloping of MLPA amplification products. Signal sloping is the effect that longer probes generate a lower peak height/area on the electropherogram than shorter probes; the exact amount of signal sloping differs between sequencer types and can differ between different samples and can disturb the analysis.

The use of P200 is recommended when a large number of synthetic probes are used, preferably targeting sequences on different chromosomes. Results can be difficult to interpret when P200 is used in combination with a small number of probes all targeting the same gene or chromosomal region, and with lengths much smaller than the reference probes. In samples with sloping, it may seem in such cases that all sequences targeted by the synthetic probes are deleted or duplicated. The use of P300 is recommended when a smaller number of synthetic probes is used. Please note that MRC Holland cannot offer support for custom probe design anymore.

Regulatory status

SALSA MLPA Probemix P200 Reference-1 is for research use only (RUO) in all territories.

Product documentation

Version B1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P200-100R
SALSA MLPA Probemix P200 Reference-1 – 100 rxn
MLPA
€ 486.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00

Other products

These optional accessories can be ordered separately.

Item no.
Description
Technology
Price
SMR50
SALSA HhaI – 115 μl
MS-MLPA
€ 48.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Resources

General MLPA resources

MLPA education

This site uses cookies to help improve your online experience. Learn more
No, thank you accept

Sign in

Don't have an account? Create one

Forgot password?

Select Your Country

Choose your country to see the products for your location