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P175 Tumour Gain

SALSA MLPA Probemix P175 Tumour Gain detects copy number variations in 24 genes frequently gained or amplified in various tumour types.

Specifications

Contents: 62 MLPA probes, including 2 probes each for ABL1, ALK, AR, AURKA, AURKB, BRAF, CCND1, CCND2, CDK4, DHFR, EGFR, ERBB2, FGFR1, KDR, KIT, MDM2, MDM4, MET, MYC, MYCN, PDGFRA, RET, SMO and TOP2A, and a probe for BRAF p.V600E (c.1799T>A).

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on various tumour types.

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

General information

SALSA MLPA Probemix P175 Tumour Gain is a research use only (RUO) assay for the detection of copy number aberrations in 24 genes, which are frequently gained or amplified in various tumour types. This probemix can also be used to detect the presence of the BRAF p.V600E (c.1799T>A) point mutation.

Regulatory status

SALSA MLPA Probemix P175 Tumour Gain is for research use only (RUO) in all territories.

SALSA Sample DNA for this product

SALSA Binning DNA SD029 is an artificial DNA sample with a signal for all probes in the P175 Tumour Gain probemix. Inclusion of a reaction with SD029 in initial experiments and in experiments following a change in electrophoresis conditions is recommended to aid in the creation of a bin set that links peaks to the probes that produce them. Binning DNA cannot be used as a reference sample in the MLPA data analysis, and cannot be used to quantify the signals of mutation-specific probes.

A vial of SALSA Binning DNA SD029 is included with every order of the P175 Tumour Gain probemix, but it is possible to order additional vials separately.

For more information, see the product description.

Product documentation

Version B2

Version B1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P175-025R
SALSA MLPA Probemix P175 Tumour Gain – 25 rxn
MLPA
€ 296.00
P175-050R
SALSA MLPA Probemix P175 Tumour Gain – 50 rxn
MLPA
€ 580.00
P175-100R
SALSA MLPA Probemix P175 Tumour Gain – 100 rxn
MLPA
€ 1135.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00

Sample DNAs (included)

A vial is included with every order of this probemix, but additional vials can also be purchased separately.

Item no.
Description
Technology
Price
SD029
SALSA Binning DNA SD029 – 6 rxn
MLPA MS-MLPA
€ 25.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

The commercially available positive samples below can be used with the current (B2) version of this product.

Germline samples

  • Coriell NA00945 (f): Heterozygous deletion affecting the probes for MYCN on chromosome 2.
  • Coriell NA01059 (f): Heterozygous deletion affecting the probes for MET on chromosome 7.
  • Coriell NA02030 (m): Heterozygous duplication affecting the probes for FGFR1 and MYC on chromosome 8.
  • Coriell NA03999 (f): Heterozygous deletion affecting the probes for MYC on chromosome 8.
  • Coriell NA05347 (m): Heterozygous duplication affecting the probes for MDM4 on chromosome 1.
  • Coriell NA07081 (m): Heterozygous duplication affecting the probes for EGFR on chromosome 7.
  • Coriell NA07412 (m): Heterozygous deletion affecting the probes for BRAF on chromosome 7.
  • Coriell NA07981 (m): Homozygous duplication / Heterozygous triplication affecting the probes for CCND2 on chromosome 12.
  • Coriell NA10401 (f): Heterozygous duplication affecting the probes for MYCN and ALK on chromosome 2. Some of the reference probes are also affected by CNAs.
  • Coriell NA12519 (f): Homozygous duplication/ Heterozygous triplication affecting the probes for MET, SMO and BRAF on chromosome 7.
  • Coriell NA13685 (f): Heterozygous duplication affecting the probes for ABL1 on chromosome 9.

Cancer cell line samples

Some of the reference probes are also affected by CNAs.

  • DSMZ ACC-427 (DU-4475) (f): Gain affecting the probes for MDM4. Positive for the BRAF p.V600E (c.1799T>A) mutation. Ambiguous ratios are observed for a gain of 7q32.1-7q34 (including MET, SMO and BRAF genes).
  • DSMZ ACC-573 (SU-DHL-8) (m): Gain affecting the probes for EGFR, MET, SMO and BRAF on chromosome 7. Gain affecting the probes for CCND2 and CDK4 on chromosome 12. Gain affecting the probes for AURKA on chromosome 20.

Resources

General MLPA resources

MLPA education

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