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P128 CYP450

SALSA MLPA Probemix P128 CYP450 detects copy number variations in the GSTM1, CYP1B1, CYP3A4, CYP3A5, CYP2C19, CYP2C9, CYP2E1, GSTP1, CYP1A2, CYP1A1, CYP2A6, CYP2B6, GSTT1 and CYP2D6 genes.

Specifications

Contents: 52 MLPA probes, including at least 2 probes for each of the targeted genes.

Tissue: human genomic DNA.

Application: research on cytochrome P450 enzymes and glutathione transferases.

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

This product is for basic research and intended for experienced users only! Interpretation of results may be complicated, and MRC Holland may only be able to provide basic support.

General information

The SALSA MLPA Probemix P128 CYP450 is a research use only (RUO) assay for the detection of deletions or duplications in the GSTM1, CYP1B1, CYP3A4, CYP3A5, CYP2C19, CYP2C9, CYP2E1, GSTP1, CYP1A2, CYP1A1, CYP2A6, CYP2B6, GSTT1 and CYP2D6 genes.

Cytochrome P450 enzymes are important in the biosynthesis and degradation of endogenous compounds such as steroids, lipids and vitamins. These enzymes reduce or alter the pharmacologic activity of many drugs and facilitate their elimination. Differences in drug metabolism in the intestine or liver between patients are major contributors to drug response, including adverse effects.

Glutathione transferases are a family of proteins that play an important role in detoxification by catalyzing the conjugation of many compounds with reduced glutathione.

In the normal population several of the targeted genes differ in copy number. More information per gene is given under Table 2 in the product description.

  • The GSTM1 gene (8 exons), spans ~5.9 kb of genomic DNA and is located on 1p13.3, 110 Mb from the p-telomere.
  • The CYP1B1 gene (3 exons), spans ~8.6 kb of genomic DNA and is located on 2p22.2, 38 Mb from p-telomere.
  • The CYP3A4 gene (13 exons), spans ~27 kb of genomic DNA and is located on 7q22.1, 99 Mb from the p-telomere.
  • The CYP3A5 gene (13 exons), spans ~32 kb of genomic DNA and is located on 7q22.1, 99 Mb from the p-telomere.
  • The CYP2C19 gene (9 exons), spans ~93 kb of genomic DNA and is located on 10q23.33, 97 Mb from the p-telomere.
  • The CYP2C9 gene (9 exons), spans ~51 kb of genomic DNA and is located on 10q23.33, 97 Mb from the p-telomere.
  • The CYP2E1 gene (9 exons), spans ~12 kb of genomic DNA and is located on 10q26.3, 135 Mb from the p-telomere (close to the q-telomere).
  • The GSTP1 gene (7 exons), spans ~2.8 kb of genomic DNA and is located on 11q13.2, 67 Mb from the p-telomere.
  • The CYP1A2 gene (7 exons), spans ~7.8 kb of genomic DNA and is located on 15q24.1, 73 Mb from the p-telomere.
  • The CYP1A1 gene (7 exons), spans ~6.0 kb of genomic DNA and is located on 15q24.1, 73 Mb from the p-telomere.
  • The CYP2A6 gene (9 exons), spans ~6.9 kb of genomic DNA and is located on 19q13.2, 46 Mb from the p-telomere.
  • The CYP2B6 gene (9 exons), spans ~27 kb of genomic DNA and is located on on 19q13.2, 46 Mb from the p-telomere.
  • The GSTT1 gene (5 exon), spans ~8.2 kb of genomic DNA and is located on 22q11.23, 23 Mb from the p-telomere.
  • The CYP2D6 gene (9 exons), spans ~4.3 kb of genomic DNA and is located on 22q13.2, 41 Mb from the p-telomere.

More information is available at https://www.ncbi.nlm.nih.gov/books/NBK1419/.

Regulatory status

SALSA MLPA Probemix P128 CYP450 is for research use only (RUO) in all territories.

Product documentation

Version D2

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P128-025R
SALSA MLPA Probemix P128 CYP450 – 25 rxn
MLPA
€ 286.00
P128-050R
SALSA MLPA Probemix P128 CYP450 – 50 rxn
MLPA
€ 560.00
P128-100R
SALSA MLPA Probemix P128 CYP450 – 100 rxn
MLPA
€ 1096.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 348.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 348.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6152.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

We have no information about specific commercially available positive samples that can be used with this product.

Resources

General MLPA resources

MLPA education

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