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P110 FCGR mix 1

SALSA MLPA probemixes P110 FCGR mix 1 and P111 FCGR mix 2 detect copy number variations in the FCGR2/3 locus.

Specifications

Contents: P110 FCGR mix 1 and P111 FCGR mix 2 together contain 78 MLPA probes for the FCGR2/3 locus, including 28 probes to detect copy number variations, and 34 probes for specific SNVs.

Tissue: genomic DNA isolated from human peripheral blood.

Application: research on the FCGR2/3 locus.

For research use only (RUO). Not for use in diagnostics.

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Key related products

P111 FCGR mix 2

Contains copy number as well as mutation specific probes for the FCGR genes. The content of P110 and P111 complement each other and we recommend to use both probemixes in order to get a more complete analysis of the FCGR region.

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This product is for basic research and intended for experienced users only! Interpretation of results may be complicated, and MRC Holland may only be able to provide basic support.

General information

SALSA MLPA Probemix P110 FCGR mix 1 and SALSA MLPA Probemix P111 FCGR mix 2 are research use only (RUO) assays for the detection of genomic rearrangements and point mutations in the 180 kb FCGR2/3 locus at 1q23.3.

Receptors for the Fc portion of IgG play an essential role in the protection of the organism against foreign antigens by removing antigen-antibody complexes from the circulation. Receptors are present on monocytes, macrophages, neutrophils, natural killer (NK) cells, and T and B lymphocytes. The receptors participate in diverse functions, such as phagocytosis of immune complexes and modulation of antibody production by B cells. Genes for several low-affinity Fc gamma receptors are clustered on chromosome 1q23.3 Within a 180 kb chromosomal area are genes for the FCGR2A, FCGR2B, FCGR2C, FCGR3A, and FCGR3B proteins. In addition, this region contains genes for the HSPA6 and HSPA7 heat shock proteins.

Due to high similarity between these FCGR genes and their close proximity, gene rearrangements are frequent in this chromosomal region. Various functionally relevant polymorphisms (SNPs) in these genes, as well as copy number variation of the FCGR2C, FCGR3A, and FCGR3B genes, have been reported. The MLPA probemixes P110 FCGR mix 1 and P111 FCGR mix 2 cover the mentioned FCGR genes and are intended to detect both copy number changes of these genes as well as frequent polymorphisms and point mutations, such as FCGR2B -386G/C, FCGR2B -120A/T, FCGR3A p.Val158Phe, FCGR2B p.Ile232Thr, FCGR2A p.His166Arg, and FCGR2A p.Gln62Trp. Probes for FCGR3B Human Neutrophil Antigen 1 (HNA1) alleles NA1, NA2 and SH, and FCGR2C STOP, classic and non-classic ORF haplotypes are also included.

Regulatory status

SALSA MLPA Probemix P110 FCGR mix 1 is for research use only (RUO) in all territories.

SALSA Sample DNA for this product

SALSA Binning DNA SD038 is an artificial DNA sample with a signal for all probes in the P110 FCGR mix 1 probemix. Inclusion of a reaction with SD038 in initial experiments and in experiments following a change in electrophoresis conditions is recommended to aid in the creation of a bin set that links peaks to the probes that produce them. Binning DNA cannot be used as a reference sample in the MLPA data analysis, and cannot be used to quantify the signals of mutation-specific probes.

A vial of SALSA Binning DNA SD038 is included with every order of the P110 FCGR mix 1 probemix, but it is possible to order additional vials separately.

For more information, see the product description.

Product documentation

Version C1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P110-025R
SALSA MLPA Probemix P110 FCGR mix 1 – 25 rxn
MLPA
€ 296.00
P110-050R
SALSA MLPA Probemix P110 FCGR mix 1 – 50 rxn
MLPA
€ 580.00
P110-100R
SALSA MLPA Probemix P110 FCGR mix 1 – 100 rxn
MLPA
€ 1135.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 361.00
EK1-CY5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 361.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK5-CY5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1658.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6373.00

Sample DNAs (included)

A vial is included with every order of this probemix, but additional vials can also be purchased separately.

Item no.
Description
Technology
Price
SD038
SALSA Reference Selection DNA SD038 – 6 rxn
MLPA
€ 25.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

We have no information about specific commercially available positive samples that can be used with this product.

Resources

General MLPA resources

MLPA education

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