Discounts on the ME001 and ME002 kits are available for specific research projects. Please enquire.
The SALSA MS-MLPA
Probemix ME001 Tumour suppressor mix 1 is a research use only (RUO)
assay for the detection of aberrant methylation of one or more sequences of 25 tumour suppressor genes (TSG).
CpG-islands are located in or near the promoter region or other regulatory regions of approximately 50% of human genes. Aberrant methylation of CpG-islands has been shown to be associated with transcriptional inactivation of genes in a wide spectrum of human cancers. These TSGs are frequently silenced by methylation in tumours, but are unmethylated in blood-derived DNA. In addition, DNA methylation analysis can indicate in some cases from which type of tissue the tumour was derived.
The SALSA MLPA Probemix ME001-D1 Tumour suppressor mix 1 contains 45 (MS-) MLPA probes with amplification products between 121 and 495 nucleotides (nt). 28 MS-MLPA probes contain a HhaI
recognition site and provide information on the methylation status of target sequence of various TSGs. All probes present will also give information on copy number changes in the analysed sample. In addition, 16 reference probes are included which are not affected by HhaI
digestion and target relatively quiet regions in terms of copy number changes in various tumour types. Also, one digestion control probe is included in this probemix indicating whether or not restriction endonuclease digestion in the MS-MLPA reaction was complete. Complete probe sequences and the identity of the genes detected by the reference probes is available in Table 2a and 2b and online (www.mlpa.com
This probemix contains nine quality control fragments generating amplification products between 64 and 121 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mlpa.com