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SALSA MLPA Probemix ME001 Tumour suppressor mix 1

Tumour suppressor genes

Region: Various

This product is for basic research and intended for experienced users only! Interpretation of results may be complicated, and MRC Holland may only be able to provide basic support.

Discounts on the ME001 and ME002 kits are available for specific research projects. Please enquire.

General information: The SALSA MS-MLPA Probemix ME001 Tumour suppressor mix 1 is a research use only (RUO) assay for the detection of aberrant methylation of one or more sequences of 25 tumour suppressor genes (TSG).

CpG-islands are located in or near the promoter region or other regulatory regions of approximately 50% of human genes. Aberrant methylation of CpG-islands has been shown to be associated with transcriptional inactivation of genes in a wide spectrum of human cancers. These TSGs are frequently silenced by methylation in tumours, but are unmethylated in blood-derived DNA. In addition, DNA methylation analysis can indicate in some cases from which type of tissue the tumour was derived.

Probemix content:
The SALSA MLPA Probemix ME001-D1 Tumour suppressor mix 1 contains 45 (MS-) MLPA probes with amplification products between 121 and 495 nucleotides (nt). 28 MS-MLPA probes contain a HhaI recognition site and provide information on the methylation status of target sequence of various TSGs. All probes present will also give information on copy number changes in the analysed sample. In addition, 16 reference probes are included which are not affected by HhaI digestion and target relatively quiet regions in terms of copy number changes in various tumour types. Also, one digestion control probe is included in this probemix indicating whether or not restriction endonuclease digestion in the MS-MLPA reaction was complete. Complete probe sequences and the identity of the genes detected by the reference probes is available in Table 2a and 2b and online (www.mlpa.com).

This probemix contains nine quality control fragments generating amplification products between 64 and 121 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mlpa.com.

Order Items

Probemix

Item no.
Description
Technology
Price
ME001-025R
SALSA MLPA Probemix ME001 Tumour suppressor mix 1 – 25 rxn
€ 260.00
ME001-050R
SALSA MLPA Probemix ME001 Tumour suppressor mix 1 – 50 rxn
€ 510.00
ME001-100R
SALSA MLPA Probemix ME001 Tumour suppressor mix 1 – 100 rxn
€ 998.00

Required Reagents (Sold Separately)

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA EK1 reagent kit – 100 rxn – FAM
€ 316.00
EK1-Cy5
SALSA MLPA EK1 reagent kit – 100 rxn – Cy5
€ 316.00
EK5-FAM
SALSA MLPA EK5 reagent kit – 500 rxn – FAM
€ 1455.00
EK5-Cy5
SALSA MLPA EK5 reagent kit – 500 rxn – Cy5
€ 1455.00
EK20-FAM
SALSA MLPA EK20 reagent kit – 2000 rxn – FAM
€ 5590.00
SMR50
SALSA HhaI – 200 rxn
€ 41.60

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SALSA MLPA Probemix ME024 9p21 CDKN2A/2B region

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SALSA MLPA Probemix P225 PTEN

Contains probes for the PTEN gene.

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