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NXtec D024 KaryoProfiler

NXtec D024 KaryoProfiler is a digitalMLPA assay for the detection of gross copy number changes, encompassing all chromosomes, typically at a ~2–4 Mb resolution.

Specifications

Contents: 1587 probes, including 1180 probes detecting copy number alterations in a genome-wide fashion, typically at a ~2–4 Mb resolution.

Tissue: human genomic DNA.

Application: research on chromosomal abnormalities associated with genetic disorders, genomic stability in iPSC, ESC and other cell models, and more (see General Information).

For research use only (RUO). Not for use in diagnostics.

Quick links

List Prices Documentation

To celebrate the launch of this product, we are offering a discount for first-time digitalMLPA users until 28 February 2026. More details.

General information

NXtec D024-A1 KaryoProfiler is a research use only (RUO) assay for the detection of gross copy number changes, encompassing all chromosomes, typically at a ~2-4 Mb resolution. Therefore, this probemix allows for fast and cost-effective genome-wide CN characterisation of DNA samples in a high-throughput fashion, allowing accurate detection of gross CN changes in various genetic backgrounds and contexts.

Possible applications may include the characterisation of DNA samples harbouring gross chromosomal abnormalities associated with genetic disorders. Such genetic aberrations may include gains or deletions of whole chromosomes, as well as gross copy number (CN) changes that are subchromosomal in nature (https://medlineplus.gov).

Gross CN determination is also pivotal in embryonic stem cell (ESC) and induced pluripotent stem cells (iPSC) characterisation, as these stem cells often acquire non-random genetic changes when cultured for a longer time. Alterations commonly include gains or losses of chromosomes or subchromosomal fragments. Frequently affected chromosomes include, but are not limited to, chromosomes 12, 17, 20 and X (The International Stem Cell Initiative, 2011; Assau et al, 2020).

Furthermore, additional applications of the NXtec D024-A1 KaryoProfiler may include:

  • Follow-up analysis of abnormal findings from FISH, array CGH, or NGS.
  • Complementation of karyotyping in cases with unclear or failed results.
  • Monitoring genomic drift and instability in long-term or high-passage cultures.
  • Screening and selection of clones prior to functional studies.
  • Detection of low-level mosaicism or subclones when present in ≥30% of the DNA sample.
  • Indication of cross-contamination, detecting contaminating cell fractions as low as 7.5%.

Sample number calculator

Use the calculator below to get an indication of the number of samples that can be included in a sequencing run. Click here if you are having issues loading or using the calculator, or if you would like more information about the calculation.

Regulatory status

NXtec D024 KaryoProfiler is for research use only (RUO) in all territories.

Product documentation

Version A1

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
D024-025R
NXtec D024 KaryoProfiler – 25 rxn
digitalMLPA
€ 605.00
D024-050R
NXtec D024 KaryoProfiler – 50 rxn
digitalMLPA
€ 1211.00
D024-100R
NXtec D024 KaryoProfiler – 100 rxn
digitalMLPA
€ 2421.00

Required reagents

A general NXtec Reagent Kit is required for digitalMLPA NXtec experiments (to be ordered separately).

Item no.
Description
Technology
Price
DRK01-IL
NXtec Reagent Kit – 100 rxn (6 vials)
digitalMLPA
€ 348.00
DRK05-IL
NXtec Reagent Kit – 500 rxn (5×6 vials)
digitalMLPA
€ 1600.00
DRK20-IL
NXtec Reagent Kit – 2000 rxn (5×6 vials)
digitalMLPA
€ 6152.00
BP01-IL
NXtec Barcode Plate 1 (barcode solutions 1–96) – 768 rxn
digitalMLPA
€ 1727.00
BP02-IL
NXtec Barcode Plate 2 (barcode solutions 97–192) – 768 rxn
digitalMLPA
€ 1727.00
BP03-IL
NXtec Barcode Plate 3 (barcode solutions 193–288) – 768 rxn
digitalMLPA
€ 1727.00
BP04-IL
NXtec Barcode Plate 4 (barcode solutions 289–384) – 768 rxn
digitalMLPA
€ 1727.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

Positive samples for this product can be found in the file D024-A1-0125 Supplemental Table 1 - positive samples.xlsx.

Resources

D024 KaryoProfiler

General digitalMLPA resources

digitalMLPA education

Publications

References

  • Assou S et al. (2020). Recurrent Genetic Abnormalities in Human Pluripotent Stem Cells: Definition and Routine Detection in Culture Supernatant by Targeted Droplet Digital PCR. Stem Cell Reports. 14:1-8.
  • International Stem Cell Initiative et al. (2011). Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Nat Biotechnol. 29:1132-44.
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New Product

First released on Nov 11, 2025