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P308 MET

SALSA® MLPA® Probemix P308 MET detects copy number variations in the MET, PTEN and LRRK2 genes.

Specifications

Contents: 48 MLPA probes, including 23 probes for MET, 4 probes for PTEN and 4 probes for LRRK2.

Tissue: human genomic DNA, including DNA from FFPE tissue.

Application: research on MET, PTEN and LRRK2.

For research use only (RUO). Not for use in diagnostics.

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List Prices Documentation

Key related products

P052 Parkinson mix 2

Contains seven probes for the LRRK2 gene.

P105 Glioma

Contains among other target genes (PDGFRA, EGFR, CDKN2A, CDK4-MIR26A2-MDM2, NFKBIA and TP53) nine probes for the PTEN gene.

P225 PTEN

Contains at least two probes for each of the 9 exons of the PTEN gene.

View all

General information

The SALSA MLPA Probemix P308 MET is a research use only (RUO) assay for the detection of deletions or duplications in the MET, PTEN and LRRK2 genes.

The receptor tyrosine kinase MET, also known as hepatocyte growth factor receptor (HGFR), is frequently amplified in human cancers. Amplification results in high cell surface densities and constitutive activation of HGFR even in the absence of growth factor stimulation by its endogenous ligand, hepatocyte growth factor (HGF). The MET gene is essential for survival and long-distance migration of epithelial and myogenic precursors during embryogenesis. Cancer cells hijack this biological process for invasion and metastasis. Multiple mechanisms can lead to aberrant MET activation, which occurs in many types of cancers.

MET gene amplifications leading to constitutive activation of the MET receptor are detected in a wide range of tumour types e.g. brain tumours, gastric cancer and lung tumours (Peters and Adjei 2012). Patients with papillary renal carcinomas carry both germline and somatic point mutations in MET (Schmidt et al. 1997; Schmidt et al. 1999). The amplification of leucine-rich repeat kinase-2 (LRRK2) is required for oncogenic MET signalling in papillary renal and in thyroid carcinomas and acts downstream of MET activation (Looyenga et al. 2011). Loss of tumour suppressor protein PTEN together with amplification of MET can be an alternative mechanism for primary resistance for tyrosine kinase inhibitors (EGFR-TKIs) (Engelman et al. 2007; Sos et al. 2009).

Regulatory status

SALSA MLPA Probemix P308 MET is for research use only (RUO) in all territories.

Product documentation

Version B3

    General documentation

Other versions

Contact us for earlier versions.

List prices

Product

Item no.
Description
Technology
Price
P308-025R
SALSA MLPA Probemix P308 MET – 25 rxn
MLPA
€ 286.00
P308-050R
SALSA MLPA Probemix P308 MET – 50 rxn
MLPA
€ 560.00
P308-100R
SALSA MLPA Probemix P308 MET – 100 rxn
MLPA
€ 1096.00

Required reagents

A general SALSA MLPA Reagent Kit is required for MLPA experiments (to be ordered separately).

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA Reagent Kit – 100 rxn – FAM (6 vials)
MLPA MS-MLPA
€ 348.00
EK1-Cy5
SALSA MLPA Reagent Kit – 100 rxn – Cy5 (6 vials)
MLPA MS-MLPA
€ 348.00
EK5-FAM
SALSA MLPA Reagent Kit – 500 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK5-Cy5
SALSA MLPA Reagent Kit – 500 rxn – Cy5 (5×6 vials)
MLPA MS-MLPA
€ 1600.00
EK20-FAM
SALSA MLPA Reagent Kit – 2000 rxn – FAM (5×6 vials)
MLPA MS-MLPA
€ 6152.00

Price details & ordering

The prices above are list prices for direct orders from MRC Holland. Contact us for a quote that takes discounts and additional costs (such as shipping costs) into account. Different prices apply for orders through one of our sales partners; contact your local supplier for a quote.

More information about ordering & prices

Positive samples

Inclusion of a positive sample is usually not required, but can be useful for the analysis of your experiments. MRC Holland has very limited access to positive samples and cannot supply such samples. We recommend using positive samples from your own collection. Alternatively, you can use positive samples from an online biorepository, such as the Coriell Institute.

The commercially available positive samples below have been tested with the current (B3) version of this product and have been shown to produce useful results.

  • Coriell NA01059: Heterozygous deletion affecting all probes for MET. Flanking probes on 7q31.2 are also affected.
  • Coriell NA12519: Homozygous duplication affecting all probes for MET. Flanking probes on 7q31.2 are also affected.
  • Coriell NA20125: Heterozygous duplication affecting all probes for PTEN.

Resources

General MLPA resources

MLPA education

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