The SALSA MLPA
Probemix P458 Gastric cancer is a research use only (RUO)
assay for the detection of deletions or duplications in 16 genes that are suggested to be of diagnostic and/or clinical relevance in gastric cancer.
Gastric cancer is an aggressive disease with an overall 5-year survival rate of ~20% and ranking second after lung cancer in annual cancer mortality. The clinical outcome of gastric cancer patients is gradually improving. However, the prognosis of patients with advanced stage is still very disappointing. A subset of gastric cancers harbour amplifications of certain receptor tyrosine kinases (RTKs) such as ERBB2
, which are associated with gastric cancer progression. Inhibitors for these RTKs are already available and could be effectively used in targeted treatments for gastric cancer patients. This probemix includes probes for the most relevant genomic alterations, which could be used as predictive biomarkers or as markers for resistance or sensitivity for targeted therapies in gastric cancer.
The SALSA MLPA Probemix P458-B1 Gastric cancer contains 61 MLPA probes with amplification products between 113 and 499 nt. This P458-B1 probemix contains 46 probes for the following genes that are suggested to be of prognostic and/or therapeutic relevance in gastric cancer: PIK3CA
(18q11.2) and CCNE1
(19q12). In addition, 15 reference probes have been included in this probemix, which target relatively quiet regions in gastric cancer. However, it should be noticed that gastric cancer karyotypes can harbour multiple numerical and structural aberrations, which can complicate interpretation of these reference probes. Complete probe sequences and the identity of the genes detected by the reference probes is available in Table 2b and online (www.mlpa.com
This probemix contains nine quality control fragments generating amplification products between 64 and 121 nt: four DNA Quantity Fragments (Q-fragments), two DNA Denaturation Fragments (D-fragments), one benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mlpa.com