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SALSA MLPA Probemix P458 Gastric cancer

Gastric cancer

Region: various

This probemix will be discontinued on 01 January 2022.

General Information: The SALSA MLPA Probemix P458 Gastric cancer is a research use only (RUO) assay for the detection of deletions or duplications in 16 genes that are suggested to be of diagnostic and/or clinical relevance in gastric cancer.

Gastric cancer is an aggressive disease with an overall 5-year survival rate of ~20% and ranking second after lung cancer in annual cancer mortality. The clinical outcome of gastric cancer patients is gradually improving. However, the prognosis of patients with advanced stage is still very disappointing. A subset of gastric cancers harbour amplifications of certain receptor tyrosine kinases (RTKs) such as ERBB2, FGFR2, KRAS and MET, which are associated with gastric cancer progression. Inhibitors for these RTKs are already available and could be effectively used in targeted treatments for gastric cancer patients. This probemix includes probes for the most relevant genomic alterations, which could be used as predictive biomarkers or as markers for resistance or sensitivity for targeted therapies in gastric cancer.

Probemix content: The SALSA MLPA Probemix P458-B1 Gastric cancer contains 61 MLPA probes with amplification products between 113 and 499 nt. This P458-B1 probemix contains 46 probes for the following genes that are suggested to be of prognostic and/or therapeutic relevance in gastric cancer: PIK3CA (3q26.32), EGFR (7p11.2), CDK6 (7q21.2), MET (7q31.2), GATA4 (8p23.1), FGFR1 (8p12), MYC (8q24.21), PTP4A3 (8q24.3), FGFR2 (10q26.13), CCND1 (11q13.2), KRAS (12p12.1), KLF5 (13q22.1), ERBB2 (17q12), TOP2A (17q21.2), GATA6 (18q11.2) and CCNE1 (19q12). In addition, 15 reference probes have been included in this probemix, which target relatively quiet regions in gastric cancer. However, it should be noticed that gastric cancer karyotypes can harbour multiple numerical and structural aberrations, which can complicate interpretation of these reference probes. Complete probe sequences and the identity of the genes detected by the reference probes is available in Table 2b and online (www.mlpa.com).

This probemix contains nine quality control fragments generating amplification products between 64 and 121 nt: four DNA Quantity Fragments (Q-fragments), two DNA Denaturation Fragments (D-fragments), one benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mlpa.com.

Order Items

Probemix

Item no.
Description
Technology
Price
P458-025R
SALSA MLPA Probemix P458 Gastric cancer – 25 rxn
€ 243.00
P458-050R
SALSA MLPA Probemix P458 Gastric cancer – 50 rxn
€ 486.00
P458-100R
SALSA MLPA Probemix P458 Gastric cancer – 100 rxn
€ 972.00

Required Reagents (Sold Separately)

Item no.
Description
Technology
Price
EK1-FAM
SALSA MLPA EK1 reagent kit – 100 rxn – FAM
€ 300.00
EK1-Cy5
SALSA MLPA EK1 reagent kit – 100 rxn – Cy5
€ 300.00
EK5-FAM
SALSA MLPA EK5 reagent kit – 500 rxn – FAM
€ 1380.00
EK5-Cy5
SALSA MLPA EK5 reagent kit – 500 rxn – Cy5
€ 1380.00
EK20-FAM
SALSA MLPA EK20 reagent kit – 2000 rxn – FAM
€ 5295.00

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SALSA MLPA Probemix P308 MET

Contains probes for each exon of MET gene et 7q31.2.

SALSA MLPA Probemix P315 EGFR

Contains probes for each exon of EGFR gene at 7p11.2.

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Contains more probes for FGFR1 gene at 8p12.

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